mTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA Kit

Product Name

mTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA Kit

See all TOR/mTOR products

SKU/Catalog Number

EKC2319

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The mTOR (Phospho-Thr2446) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor mTOR (Phospho-Thr2446) protein expression profile in cells. The kit can be used for measuring the relative amounts of mTOR (Phospho-Thr2446) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on mTOR (Phospho-Thr2446).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

mTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2319)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2319 is reactive to MTOR in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2319 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	2 Plates	-
10x TBS	24 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	50 ml (15x)	Clear
100x Anti-mTOR Phospho-Thr2446 Antibody (Rabbit Polyclonal)	60 µl (100x)	Red
100x Anti-mTOR Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	12ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	12ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	12ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For MTOR (Source: Uniprot.Org, NCBI)

Alternative Names

FRAP;FRAP1;FRAP2;RAFT1;Rapamycin target protein;kinase mTOR MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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