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Product Info Summary
| SKU: | P00003 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00003
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody catalog # P00003. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00003)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-mTOR (S2448)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00003 is reactive to MTOR in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
53 kDa
Calculated molecular weight
288892 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00003 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
Positive Control
WB: HEK293 cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-mTOR (S2448) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MTOR (Source: Uniprot.org, NCBI)
Gene Name
MTOR
Full Name
Serine/threonine-protein kinase mTOR
Weight
288892 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTOR, check out the MTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody (P00003)
Hello CJ!
P00003 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xia Y,Zhang G,Han C,Ma K,Guo X,Wan F,Kou L,Yin S,Liu L,Huang J,Xiong N,Wang T.Microglia as modulators of exosomal alpha-synuclein transmission.Cell Death Dis.2019 Feb 20;10(3):174.doi:10.1038/s41419-019-1404-9.PMID:30787269;PMCID:PMC6382842.
Species: Human,Mouse
P00003 usage in article: APP:WB, SAMPLE:BV2 CELL, DILUTION:1:400
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
P00003 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Shao CS,Zhou XH,Zheng XX,Huang Q.Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells.J Ethnopharmacol.2020 Nov 15;262:113213.doi:10.1016/j.jep.2020.113213.Epub 2020 Aug 2.PMID:32755651.
Species: Human
P00003 usage in article: APP:WB, SAMPLE:EC9706 CELLS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Question
We have been able to see staining in mouse testis. What should we do? Is anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
From literature testis does express MTOR. From Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody P00003 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, pig. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody (P00003) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-23
Question
We ordered your anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody for IHC on liver in the past. I am using mouse, and I plan to use the antibody for WB next. I am looking for examining liver as well as b-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
I viewed the website and datasheets of our anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody and it seems that P00003 has been validated on mouse in both IHC and WB. Thus P00003 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-17
Question
Can P00003 be used on porcine?
Verified customer
Asked: 2019-04-12
Answer
The Anti-Phospho-MTOR (S2448) Rabbit Monoclonal Antibody (P00003) hasn't been tested on porcine, we are uncertain if the antibodies are suitable for porcine samples. The immunogen sequence is CSRTRTD-pS-YSAG.
Boster Scientific Support
Answered: 2019-04-14
Question
My team were satisfied with the WB result of your anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?
B. Moore
Verified customer
Asked: 2017-03-21
Answer
From what I have seen in literature, liver does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-03-21
Question
We need using your anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody for vegfr2 mediated vascular permeability studies. Has this antibody been tested with western blotting on breast cancer cells? We would like to see some validation images before ordering.
N. Krishna
Verified customer
Asked: 2015-08-17
Answer
We appreciate your inquiry. This P00003 anti-Phospho-mTOR (S2448) Rabbit Monoclonal antibody is validated on breast cancer cells. It is guaranteed to work for IHC, WB in human, mouse, pig. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-08-17
