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Product Info Summary
| SKU: | M00221 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LRRK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00221
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LRRK2 Rabbit Monoclonal Antibody catalog # M00221. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRRK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00221)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BBE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LRRK2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00221 is reactive to LRRK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
286103 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00221 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of LRRK2 in HEK293 cell lysate transfected with 3*Flag wild type, full length LRRK2.
Protein Target Info & Infographic
Gene/Protein Information For LRRK2 (Source: Uniprot.org, NCBI)
Gene Name
LRRK2
Full Name
Leucine-rich repeat serine/threonine-protein kinase 2
Weight
286103 MW
Superfamily
protein kinase superfamily
Alternative Names
Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8; Lrrk2|4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-4, cI-46|leucine-rich repeat kinase 2|leucine-rich repeat serine/threonine-protein kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRK2, check out the LRRK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRK2 Rabbit Monoclonal Antibody (M00221)
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Customer Q&As
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15 Customer Q&As for Anti-LRRK2 Rabbit Monoclonal Antibody
Question
I see that the anti-LRRK2 Rabbit Monoclonal antibody M00221 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-29
Question
I am interested in to test anti-LRRK2 Rabbit Monoclonal antibody M00221 on rat liver for research purposes, then I may be interested in using anti-LRRK2 Rabbit Monoclonal antibody M00221 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The products we sell, including anti-LRRK2 Rabbit Monoclonal antibody M00221, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-LRRK2 Rabbit Monoclonal antibody M00221 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
M. Mitchell
Verified customer
Asked: 2020-01-23
Answer
The anti-LRRK2 Rabbit Monoclonal antibody (M00221) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-23
Question
Our lab were content with the WB result of your anti-LRRK2 Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
From what I have seen in literature, liver does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-12-09
Question
Is this M00221 anti-LRRK2 Rabbit Monoclonal antibody reactive to the isotypes of LRRK2?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of M00221 anti-LRRK2 Rabbit Monoclonal antibody is A synthesized peptide derived from human LRRK2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
I was wanting to use your anti-LRRK2 Rabbit Monoclonal antibody for ICC for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
As indicated on the product datasheet, M00221 anti-LRRK2 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-18
Question
Can you help my question with product M00221, anti-LRRK2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00221 anti-LRRK2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-14
Question
Do you have a BSA free version of anti-LRRK2 Rabbit Monoclonal antibody M00221 available?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LRRK2 Rabbit Monoclonal antibody M00221 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-03
Question
Is a blocking peptide available for product anti-LRRK2 Rabbit Monoclonal antibody (M00221)?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
We do provide the blocking peptide for product anti-LRRK2 Rabbit Monoclonal antibody (M00221). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-22
Question
See below the WB image, lot number and protocol we used for liver using anti-LRRK2 Rabbit Monoclonal antibody M00221. Please let me know if you require anything else.
C. Lewis
Verified customer
Asked: 2018-01-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-05
Question
Would M00221 anti-LRRK2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
As indicated on the product datasheet, M00221 anti-LRRK2 Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-26
Question
We have observed staining in rat testis. What should we do? Is anti-LRRK2 Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
From literature testis does express LRRK2. From Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2017-08-07
Question
We bought anti-LRRK2 Rabbit Monoclonal antibody for IP on brain last year. I am using human, and We want to use the antibody for IHC next. I am looking for examining brain as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
B. Lewis
Verified customer
Asked: 2017-04-25
Answer
I viewed the website and datasheets of our anti-LRRK2 Rabbit Monoclonal antibody and I see that M00221 has been validated on human in both IP and IHC. Thus M00221 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-LRRK2 Rabbit Monoclonal antibody M00221. Let me know if you need anything else.
Z. Brown
Verified customer
Asked: 2017-02-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-14
Question
Does anti-LRRK2 Rabbit Monoclonal antibody M00221 work for ICC with liver?
G. Baker
Verified customer
Asked: 2015-03-27
Answer
According to the expression profile of liver, LRRK2 is highly expressed in liver. So, it is likely that anti-LRRK2 Rabbit Monoclonal antibody M00221 will work for ICC with liver.
Boster Scientific Support
Answered: 2015-03-27
