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Product Info Summary
| SKU: | P00221 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00221
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody catalog # P00221. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00221)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BFH-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-LRRK2 (S935)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00221 is reactive to LRRK2 in Human, Mouse
Reconstitution
Observed Molecular Weight
100 kDa
Calculated molecular weight
286103 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00221 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
Positive Control
WB: WT-LRRK2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Filamin A phosphorylation expression in WT-LRRK2 cell lysate treated LRRK2.
Protein Target Info & Infographic
Gene/Protein Information For LRRK2 (Source: Uniprot.org, NCBI)
Gene Name
LRRK2
Full Name
Leucine-rich repeat serine/threonine-protein kinase 2
Weight
286103 MW
Superfamily
protein kinase superfamily
Alternative Names
Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8; Lrrk2|4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-4, cI-46|leucine-rich repeat kinase 2|leucine-rich repeat serine/threonine-protein kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRK2, check out the LRRK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody (P00221)
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No publications found for P00221
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for visceral pleura using anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
My boss were well pleased with the WB result of your anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody. However we have seen positive staining in brain cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From what I have seen in literature, brain does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody (P00221) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
Question
Will P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It shows on the product datasheet, P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-02
Question
Is a blocking peptide available for product anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody (P00221)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We do provide the blocking peptide for product anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody (P00221). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-15
Question
We need to test anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 on mouse visceral pleura for research purposes, then I may be interested in using anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The products we sell, including anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-04
Question
We ordered your anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody for WB on testis a few years ago. I am using mouse, and We want to use the antibody for IF next. We need examining testis as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
I have checked the website and datasheets of our anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody and I see that P00221 has been validated on mouse in both WB and IF. Thus P00221 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-20
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
From literature liver does express LRRK2. From Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-08-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
Is this P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody reactive to the isotypes of LRRK2?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The immunogen of P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody is A synthesized peptide derived from human Phospho-LRRK2 (S935) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-21
Question
Is there a BSA free version of anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 available?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-17
Question
Does anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 work for WB with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
According to the expression profile of visceral pleura, LRRK2 is highly expressed in visceral pleura. So, it is likely that anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 will work for WB with visceral pleura.
Boster Scientific Support
Answered: 2019-03-21
Question
I was wanting to use your anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody for WB for mouse visceral pleura on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse visceral pleura identification?
B. Taylor
Verified customer
Asked: 2017-12-21
Answer
It shows on the product datasheet, P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-21
Question
I have a question about product P00221, anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00221 anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-24
Question
I see that the anti-Phospho-LRRK2 (S935) Rabbit Monoclonal antibody P00221 works with WB, what is the protocol used to produce the result images on the product page?
E. Anderson
Verified customer
Asked: 2016-04-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-04-22
