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IHC Technical Resources
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IHC Principle
Western blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.
IHC is the application of the immunoassay principle to tissue staining. By using antibodies to direct staining, IHC can accurately detect protein localization while generating crisp stains of tissue structures.
Click Here for MoreIHC Sample Preparation
Every immunoassay protocol begins with sample preparation. Western blot, IHC, and ELISA all require different ways of preparing a sample, and nearly every sample type requires specialized treatment in order to produce high-quality, consistent results.
IHC, unlike Western blot or ELISA, does not require that proteins be extracted from cells or tissues. Instead, sample preparation for IHC focuses on properly fixing, slicing, and mounting the samples. Use these sample preparation guides to learn how to prepare cell cultures and various tissue types for IHC.
Click Here for MoreIHC Protocol
The Boster Bio protocol for IHC offers a step-by-step overview of the procedure. Use this guide as a primer or a quick reference guide, and see our product datasheets or sample preparation guides for more detailed information.
Click Here for MoreIHC Optimization Tips
Nearly every step of the IHC procedure comes with a choice. Which sample preparation method is best? How concentrated should the antibodies be? Which blocking buffer will work best?
The Boster Bio optimization tips are meant to help answer those questions and more. Take a look at our optimization guides to help inform your decisions, optimize your experiments, and get better results.
Click Here for MoreIHC Troubleshooting Tips
Every researcher eventually encounters trouble with an experiment. Blots appear blank, or IHC stains come out blurry, or ELISA standard curves come out wonky, or any of a thousand different problems.
Proper controls can help eliminate a great number of possible sources of error, but many troubles will remain. Check out the Boster troubleshooting guides for the most common troubles, their sources, and how to resolve them.
Click Here for MoreTake your immunohistochemistry to the next level with multiplexing capabilities! Our innovative multiplex IHC solutions empower you to efficiently analyze multiple protein targets in a single experiment, saving time and resources while maximizing data output.
Related Pages
Immunohistochemistry (IHC) Fundamental Principle, How IHC Works
The principle behind Immunohistochemistry (IHC) entails detection of antigen or happens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Learn more about this in this guide.
See MoreImmunohistochemistry (IHC) Optimization Tips
IHC optimization is a critical step in any test. This guide gives you insight on antigen retrieval, fixation and embedding. Learn how to optimize your immunohistochemistry test to get valuable results.
See MoreImmunohistochemistry (IHC) Troubleshooting Guide
This guide provides a thorough list of Immunohistochemistry troubleshooting tips, including weak staining, high background, nonspecific staining among others. Learn how to take control of your IHC process.
See MoreAll Technical Support Resources
Boster provides comprehensive technical resources for applications, blogs, research area/pathway map/disease information, and web-based digital tools.
See MoreImmunohistochemistry Troubleshooting Guide Download Page
This guide will show you all the nuts and blots for Immunohistochemistry (IHC), including expert review of principle, optimized protocol that really works and more. Comprehensive Troubleshooting Tips Having our troubleshooting tips that address all k...
See MoreIHC and ICC/IF Sample Preparation
Learn the best IHC and ICC sample preparation techniques. Get a detailed procedure of preparing different types of preserved tissues which is key to getting high quality staining during Immunohistochemistry (IHC).
See MoreImmunohistochemistry IHC Reagents
Tissue Preparation Tissue preparation is an important step in IHC as the antigen exhibition and location are largely dependent on the quality of the tissue sample. Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells or t...
See MoreIHC Controls You Should Know
After saturation, the inactivated antibody replaces the primary antibody in the IHC protocol for the control. The samples are incubated with only the antibody diluent without adding the primary antibody. No Primary Controls No primary controls (aka s...
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