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Flow Cytometry Optimization
For a more comprehensive Flow Cytometry optimization guide, download our how to become Flow Cytometry expert eBook
Download Flow Cytometry ebookSample Preparation Optimization
The first step to obtaining high quality flow data is sample preparation. The critical step of preparing a single cell suspension will help to avoid unwanted instrument clogs and low quality data. Use this guide for tips on how to optimize your sample preparation method and start getting better results.
Keywords: flow cytometry optimization, sample preparation, FACS experiment sample prep
Learn More About Sample Prep OptimizationPermeabilization And Fixation Method Optimization
Various permeabilization and fixation methods are available to allow access of antibodies to intracellular proteins. Fixing cells will retain the target protein in its original cellular location, and will usually ensure better stability of soluble antigens and antigens with a short half-life. Use this guide to help decide which method is optimal for your experiment.
Keywords: FACS, flow cytometry, optimization, permeabilization, fixation method, fixative
Learn More About Permeabilization and Fixation OptimizationExperimental Controls Optimization
Flow experiments can become very complex with multiple parameters and mixed cell populations, and sometimes there can be numerous experimental controls involved. Use this guide to help clarify and choose appropriate controls for your FACS experiment.
Keywords: flow cytometry, FACS experimental controls, negative and positive control, isotype, FMO controls
Learn More About Controls OptimizationFluorescent Conjugates (Fluorophore) Optimization
There are many fluorescent molecules, also known as fluorochromes, fluorophores, or fluorescent dyes, with a potential application in flow cytometry. By conjugating (pre-attaching) them to primary antibodies, we can create conjugated antibodies that allow for flow cytometry analysis. Use this guide to learn how to choose the right fluorescent labels for your FACS experiment.
Keywords: Fluorochrome selection, fluorophores, fluorescent dyes, labels, tags, molecules, fluorescent stain
Learn More About Fluorochrome OptimizationCell Stimulation Optimization (For Intracellular Cytokine Staining)
To optimize your flow experiment, additional in vitro cell stimulation may be required to trigger increased production of intracellular cytokines. Some common reagents for this purpose include PMA, Ca++ or peptide epitopes and protein transport inhibitor, Brefeldin A, and more. Use this guide to learn the optimal method for stimulating your cell samples before flow cytometric analysis.
Keywords: optimize in vitro cell stimulation, increase intracellular cytokine response
Learn More About Cell Stimulation OptimizationAntibody Immuno-Staining Optimization
The antibody staining procedure includes many steps, such as antibody titration, washing, blocking, and more. The optimal antibody concentration, which gives the best staining with minimum background, must be determined experimentally. Use this guide for tips on how to optimize the steps of your antibody staining protocol.
Keywords: FACS experiment optimization, antibody staining, titration, fluorescent staining
Learn More About Fluorescence Staining OptimizationFlow Cytometry Technical Resources
Protocols, optimization tips, troubleshooting guides, and more for flow cytometry.
Technical ResourcesTroubleshooting Guides
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.
Troubleshooting GuidesRelated Pages
Flow Cytometry (FACS) Troubleshooting Guide
Boster Bio is an antibody company and supplier. Read more about our troubleshooting tips for flow cytometry (FACS) experiments. Learn tips on how to resolve issues such as high background, and weak or no signal
See MoreFlow Cytometry Fundamental Principle, How FACS Works
Flow cytometry is a popular cell biology technique that utilizes laser-based technology to count, sort, and profile cells in a heterogeneous fluid mixture. FACS is an abbreviation for fluorescence-activated cell sorting, which is a flow cytometry technique that further adds a degree of functionality.
See MoreFlow Cytometry Protocols
Boster Bio protocols for flow cytometry offer a step-by-step overview of the procedure. Use this guide as a primer or a quick reference guide, and see our product datasheets or sample preparation guides for more details.
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