This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
, No Gclids
ELISA Optimization
For a more comprehensive ELISA optimization guide, download our how to become ELISA expert eBook.
Download ELISA ebookOutsource your ELISA Experiment Now!
Boster provides ELISA CRO services in a simple process: send us your samples, we test them for your analytes of interest and send you the results data.
ELISA Blocking Buffer Optimization
There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specific application.
Keywords: ELISA, optimization, blocking, buffer, protein blocker, detergent blocker
Learn More About Blocking OptimizationELISA Plate Washing Optimization
One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and quantify nearly any molecule. Optimizing the parameters of the wash steps is critical to obtaining the best results from your ELISA assay. Use this guide to help decide how you will optimize the parameters of your washes:
Keywords: ELISA, optimization, antibody, antigen, molecules, ELISA assay, sample, concentration, plate washing, wash parameters
Learn More About Washing OptimizationELISA Antibody/Antigen Concentration Optimization
In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies.
Keywords: ELISA, optimization, antibody, antigen, sample, concentration, checkerboard, titration
Learn More About Checkerboard TitrationELISA Detection System Optimization
The choice of which detection system to use is influenced by the equipment available and the needs of the specific application being considered. Use this guide to help decide whether to use enzyme-linked secondary antibodies, or fluorescently labelled secondary antibodies as your detection system.
Keywords: ELISA, optimization, enzyme, fluorescent, enzyme, conjugate, secondary antibodies
Learn More About Detection SystemsELISA Technical Resources
Protocols, optimization tips, troubleshooting guides, and more for ELISA.
Technical ResourcesTroubleshooting Guides
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.
Troubleshooting GuidesRelated Pages
Picokine ELISA Kit Troubleshooting
Get to know some of ELISA troubleshooting tips with this guide. It has some commonly encountered problems and solutions to ELISA like a weak signal, high background, and low sensitivity. Check it out to resolve the most encountered problems in ELISA.
See MoreELISA Protocols
Learn stepwise ELISA protocols from reagent preparation to data analysis. Check out their differences to learn how they compare and their advantages and disadvantage.
See MoreImmunohistochemistry (IHC) Optimization Tips
IHC optimization is a critical step in any test. This guide gives you insight on antigen retrieval, fixation and embedding. Learn how to optimize your immunohistochemistry test to get valuable results.
See MoreELISA Troubleshooting Guide Download Page
This guide will teach you everything you need to become an ELISA expert, including critical review of principle, all-in-one FAQs, and more. We have everything you need to know from sample preparation to data analysis. 150+ Troubleshooting Advices Hav...
See MoreELISA Troubleshooting: Saturated Signals
Plate sealers not used or re-used During incubations, cover plates with plate sealers; Use a fresh sealer every time the used sealer is removed from the plate. Plate read at incorrect detection wavelength Use recommended wavelength/filter; Ensure pla...
See More