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Product Info Summary
| SKU: | PA1988 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TORC2/CRTC2 Antibody Picoband®
SKU/Catalog Number
PA1988
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TORC2/CRTC2 Antibody catalog # PA1988. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TORC2/CRTC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1988)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1988 is reactive to CRTC2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
73302 MW
Background of TORC2
CREB regulated transcription coactivator 2, also known as CRTC2, is a protein which in humans is encoded by the CRTC2 gene. This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1988 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human 293T whole cell, human MOLT-4 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CRTC2 using anti-CRTC2 antibody (PA1988).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRTC2 antigen affinity purified polyclonal antibody (Catalog # PA1988) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRTC2 at approximately 80 kDa. The expected band size for CRTC2 is at 73 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CRTC2 (Source: Uniprot.org, NCBI)
Gene Name
CRTC2
Full Name
CREB-regulated transcription coactivator 2
Weight
73302 MW
Superfamily
TORC family
Alternative Names
CREB-regulated transcription coactivator 2;Transducer of regulated cAMP response element-binding protein 2;TORC-2;Transducer of CREB protein 2;CRTC2;TORC2; Crtc2|4632407F12Rik, Torc2|CREB regulated transcription coactivator 2|CREB-regulated transcription coactivator 2|TORC-2|transducer of CREB protein 2|transducer of regulated cAMP response element-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRTC2, check out the CRTC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRTC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TORC2/CRTC2 Antibody Picoband® (PA1988)
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Customer Q&As
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5 Customer Q&As for Anti-TORC2/CRTC2 Antibody Picoband®
Question
Does anti-TORC2/CRTC2 antibody PA1988 work on goat WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not tested anti-TORC2/CRTC2 antibody PA1988 on goat. You can run a BLAST between goat and the immunogen sequence of anti-TORC2/CRTC2 antibody PA1988 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
Is this PA1988 anti-TORC2/CRTC2 antibody reactive to the isotypes of CRTC2?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
The immunogen of PA1988 anti-TORC2/CRTC2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2(595-611aa QDPHTFNHQNLTHCSRH), different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-29
Question
We are currently using anti-TORC2/CRTC2 antibody PA1988 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
The anti-TORC2/CRTC2 antibody (PA1988) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-29
Question
Do you have a BSA free version of anti-TORC2/CRTC2 antibody PA1988 available?
H. Carter
Verified customer
Asked: 2015-02-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TORC2/CRTC2 antibody PA1988 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-TORC2/CRTC2 antibody PA1988. Let me know if you need anything else.
J. Li
Verified customer
Asked: 2014-04-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-11
