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Product Info Summary
| SKU: | PB9281 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LRRK2 Antibody Picoband®
SKU/Catalog Number
PB9281
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRRK2 Antibody Picoband® catalog # PB9281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRRK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9281)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9281 is reactive to LRRK2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
286 kDa
Calculated molecular weight
286103 MW
Background of Lrrk2
Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is anenzyme that in humans is encoded by the PARK8 gene. It is a member of the leucine-rich repeat kinase family. This gene is mapped to 12q12. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. It has been determined that LRRK2 possesses mixed-lineage kinase activity, and LRRK2 also showed autophosphorylation activity. What’s more, LRRK2 has an affinity for lipids or lipid-associated proteins and may play a role in the biogenesis or regulation of membranous intracellular structures in the brain. It is also an IFNG target gene that may be involved in signaling pathways relevant to Crohn disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9281 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, Rat Liver Tissue, U87 Whole Cell, NEURO Whole Cell, A549 Whole Cell, SMMC Whole Cell
Validation Images & Assay Conditions
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Anti-LRRK2 Picoband antibody, PB9281, Western blotting
All lanes: Anti LRRK2 (PB9281) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: NEURO Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 286KD
Observed bind size: 286 KD
Protein Target Info & Infographic
Gene/Protein Information For LRRK2 (Source: Uniprot.org, NCBI)
Gene Name
LRRK2
Full Name
Leucine-rich repeat serine/threonine-protein kinase 2
Weight
286103 MW
Superfamily
protein kinase superfamily
Alternative Names
Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8; Lrrk2|4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-4, cI-46|leucine-rich repeat kinase 2|leucine-rich repeat serine/threonine-protein kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRK2, check out the LRRK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRK2 Antibody Picoband® (PB9281)
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No publications found for PB9281
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-LRRK2 Antibody Picoband®
Question
Would PB9281 anti-LRRK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
As indicated on the product datasheet, PB9281 anti-LRRK2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-14
Question
Is this PB9281 anti-LRRK2 antibody reactive to the isotypes of LRRK2?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of PB9281 anti-LRRK2 antibody is E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
Please see the WB image, lot number and protocol we used for brain using anti-LRRK2 antibody PB9281. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
I would like using your anti-LRRK2 antibody for canonical wnt signaling pathway studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Thanks for your inquiry. This PB9281 anti-LRRK2 antibody is validated on rat liver tissue, brain tissue, tissue lysate, mouse brain, u87 whole cell lysate, neuro whole cell lysate, a549 whole cell lysate, smmc whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-07
Question
Do you have a BSA free version of anti-LRRK2 antibody PB9281 available?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LRRK2 antibody PB9281 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-26
Question
My colleagues were satisfied with the WB result of your anti-LRRK2 antibody. However we have observed positive staining in liver cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
From what I have seen in literature, liver does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-08-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-LRRK2 antibody PB9281. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-LRRK2 antibody PB9281 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-LRRK2 antibody (PB9281) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
I have a question about product PB9281, anti-LRRK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9281 anti-LRRK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-05
Question
Is a blocking peptide available for product anti-LRRK2 antibody (PB9281)?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
We do provide the blocking peptide for product anti-LRRK2 antibody (PB9281). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-21
Question
I am looking for to test anti-LRRK2 antibody PB9281 on rat brain for research purposes, then I may be interested in using anti-LRRK2 antibody PB9281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The products we sell, including anti-LRRK2 antibody PB9281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-27
Question
Does anti-LRRK2 antibody PB9281 work for WB with brain?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
According to the expression profile of brain, LRRK2 is highly expressed in brain. So, it is likely that anti-LRRK2 antibody PB9281 will work for WB with brain.
Boster Scientific Support
Answered: 2017-10-30
Question
We have observed staining in rat testis. Are there any suggestions? Is anti-LRRK2 antibody supposed to stain testis positively?
S. Miller
Verified customer
Asked: 2016-01-15
Answer
According to literature testis does express LRRK2. According to Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2016-01-15
Question
I was wanting to use your anti-LRRK2 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
A. Carter
Verified customer
Asked: 2015-11-19
Answer
It shows on the product datasheet, PB9281 anti-LRRK2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-19
Question
I see that the anti-LRRK2 antibody PB9281 works with WB, what is the protocol used to produce the result images on the product page?
K. Yang
Verified customer
Asked: 2013-04-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-29
