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Boster Bio Custom Monoclonal Antibody Production Services
Custom Monoclonal Antibody Production Services (High Affinity Antibody Guarantee)
Expertise in Custom Monoclonal Antibody Development Services for High-Specificity Applications
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Why Use Boster for your custom antibody projects
Boster Bio stands out as a premier provider of monoclonal antibody services, with over three decades of proven expertise.
Our 25+ years of experience in antibody design and production will ensure you the best chance of getting a high affinity monoclonal antibody that works for your application. Since 1993, our team has produced over 50,000 antibodies. All you need to provide is your target sequence and Boster gets to work.
Our dedicated team of experts will guide you through every step of the antibody production process, from antigen design to the final purification of antibodies. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to drop us an email at [email protected] and we'll make sure you stay informed.
What Makes A Good Antibody?
Boster Antigen design and expression overview
Design
We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modification, secondary/tertiary structure and epitope localization.
Expression
We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.
Monoclonal Antibody Production Services OVERVIEW
Contact us at [email protected] for a formal quote.
Antigen Expression
Design & express recombinant antigen
Immunization & test-bleed on ELISA
immunize 3 mice
Fusion & Screening
Harvest splenocytes for hybridoma fusion & Screen for positive clones
Subcloning
Subclone & isolate stable positive clones
Purification
Purify and test antibodies
Deliverables
2mg monoclonal antibodies, 6-month cell banking service
Monoclonal Antibody Service PROJECT TIMELINE
Below is a general timeline of a custom mouse monoclonal antibody project. For any questions, please contact [email protected]
| Milestone | Start Day | Description | Deliverables |
|---|---|---|---|
| Pre-immune sera collection (optional) | Day 0 | (Optional) Pre-immune bleed 10ul per mouse. | |
| Plasmid synthesis | Day 1 | Synthesizing your antigen expression plasmid. | |
| Recombinant antigen expression | Day 14 | Express your protein antigen recombinantly. | Report 1: Protein expression report (Optional) Pre-immune screening report |
| Immunization preparation | Day 35 (Day 1 if you provide antigen) | Preparing antigen with CFA or IFA. Preparing mice. | |
| Immunization 1st shot | Day 52 | 3 mice, 0.5mg of antigen total per mouse | |
| Immunization 2nd shot | Day 73 | Immune boost injections, 0.25mg of antigen total per mouse. | |
| Immunization 3rd shot | Day 87 | Immune boost injections, 0.25mg of antigen total per mouse. | |
| Immunization 4th shot | Day 101 | Immune boost injections, 0.25mg of antigen total per mouse. | |
| Test bleed on ELISA | Day 106 | On the 5th day of the last immune booster shot, bleed the mice and test sera via ELISA. Decide to continue or terminate project based on the ELISA result and (optional) specific application test result. | Report 2: Poly-antisera ELISA report (Optional) Application test report, if the guaranteed specific application can be tested in house. |
| Fusion and screening | Day 108 | Fuse the splenocytes of the mouse/mice with positive anti-sera with proprietary cell line and create hybridoma cells. Screen for positive clones. | Report 3: Fusion clones ELISA report (Optional) The Recipient can order supernatant of cell culture to be tested at any time. |
| Subcloning | Day 136 | Subcloning, isolate stable positive clones. | |
| Expansion, purification & final testing | Day 157 | Expand the selected cell lines. | Report 3: Fusion clones ELISA report (Optional) The Recipient can order supernatant of cell culture to be tested at any time. |
| Deliver report, antibodies, and cell lines | Day 192 | The 2 clones and 2mg of antibodies per clone are prepared ready to be shipped to the Recipient. Note the shipping cost is not included in the project pricing. The preparation process can take 7-14 business days. | Report 4: Final delivery report, 2mg of purified monoclonal antibodies, 6 month of cell banking service (Optional) Additional clones, additional antibodies, scale up production |
Additional Services for Custom Monoclonal Antibody Development
Pre-Immune Screening
Order a vial of pre-immune serum for screening existing immunity against the target protein, and for control purposes. The pre-immune serum can be used to screen for preexisting immunity in the host, which is common for antigens originating from naturally occurring pathogens.
Price: $100 per mouse, 10ul of serum, not purified
Test Bleeds
Order test bleeds throughout the immunization process to monitor the antibody's performance on your application of interest.
Price: $100 per mouse, 10ul of serum, not purified
Additional Mice
Immunize more mice than the default three. This could increase the chance of having at least one strong positive mouse.
Price: $80 per mouse
Cell Culture Supernatants for Testing
Order cell culture supernatants to be shipped to you for testing.
Price: $100 processing fee for one shipment with 1 vial of 100ul supernatant, $10 for each additional 100ul of supernatant
Hybridoma Sequencing
Clone and sequence hybridoma antibody heavy and light chain variable regions.
Price: $800 per clone
Additional Clones
Select more clones than the default two.
Price: $250 for each additional clone
Cell Banking Service
Store clones beyond the default 6 months of free cell banking service.
Price: $30 per month per clone
Antibody Conjugation
For purified monoclonal antibodies only. Available conjugates: FITC, TRITC, Cy3, DyLight®488, Alexa®488, Colloidal Gold (5nm/10nm), Biotin, HRP.
Price: $600 per 1mg of antibodies
Blocking Peptide
If you require a blocking peptide for your developed antibody, we can help you with that.
Price: $150 for 1mg of blocking peptide
Recombinant Protein
If you want the recombinant protein we generated for you, you can purchase it from us.
Price: $200 for 1mg of purified recombinant protein
Specific Application Insurance/Additional Validation
If you would like the antibody be guaranteed for usage in certain applications, you can purchase this insurance which will give you a full refund if the final antibody is not working for your application. By default the antibody is guranteed to work in direct and indirect ELISA.
Price: Custom, depending on the application and nature of the target protein
Additional Antibodies/Scale Up Production
If you want to scale up and produce more of the monoclonal antibody we offer ascites antibody production for your chosen clone. On average, one mouse produces 2-4ml ascites. 1ml ascites contain 1mg+ monoclonal antibodies.
Price: For 1-10 mice, $30/mouse.
For 10+ mice, $20/mouse. Ascites from these productions will be delivered.
Purification cost: $30 per mouse
Intellectual Property & Exclusivity
If you intend to have exclusive rights for the produced antibodies, we can create a license that fits your needs.
Price: Custom, depending on the intended use of the antibody
Unlock the power of custom peptide synthesis now and open doors to groundbreaking discoveries.
Other Antibody Tests & Services
Custom Antibody Production:
- Rabbit Polyclonal Antibodies
- Special Deals for Custom Antibody
- Rabbit Monoclonal Antibodies
- Mouse Monoclonal Antibodies
More Customized Antibody Services We Provide
High Affinity Antibody Guarantee
Both the test bleed and the final delivery of antibodies are affinity guaranteed high affinity—we guarantee that at 1:1000 dilution ration the antibody can detect as low as 1ng of the protein antigen, or 100ng of the peptide antigen, in a direct ELISA.
Partners & Customers
Have Questions?
Feel free to contact us to discuss details about your custom antibody project.
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