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Product Info Summary
| SKU: | M00159 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-CDK4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00159
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00159)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00159 is reactive to CDK4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated molecular weight
33730 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00159 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human K562 whole cell, rat brain tissue, rat pancreas tissue, mouse brain tissue, mouse pancreas tissue
ICC/IF: NIH/3T3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (M00159).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat pancreas tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified monoclonal antibody (Catalog # M00159) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
Click image to see more details
Immunofluorescent analysis of NIH/3T3 cells, using CDK4 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)
Gene Name
CDK4
Full Name
Cyclin-dependent kinase 4
Weight
33730 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 4;2.7.11.22;Cell division protein kinase 4;PSK-J3;CDK4; CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK4, check out the CDK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK4 Rabbit Monoclonal Antibody (M00159)
Hello CJ!
M00159 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
M00159 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells
Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.
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Customer Q&As
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16 Customer Q&As for Anti-CDK4 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CDK4 Rabbit Monoclonal antibody for IP for mouse right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right ovary identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
You can see on the product datasheet, M00159 anti-CDK4 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
We have seen staining in human muscle. Are there any suggestions? Is anti-CDK4 Rabbit Monoclonal antibody supposed to stain muscle positively?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Based on literature muscle does express CDK4. Based on Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-15
Question
See below the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
I have a question about product M00159, anti-CDK4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00159 anti-CDK4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
Will anti-CDK4 Rabbit Monoclonal antibody M00159 work for IP with right ovary?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
According to the expression profile of right ovary, CDK4 is highly expressed in right ovary. So, it is likely that anti-CDK4 Rabbit Monoclonal antibody M00159 will work for IP with right ovary.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-CDK4 Rabbit Monoclonal antibody M00159 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-CDK4 Rabbit Monoclonal antibody (M00159) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
Question
Would M00159 anti-CDK4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
As indicated on the product datasheet, M00159 anti-CDK4 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Is this M00159 anti-CDK4 Rabbit Monoclonal antibody reactive to the isotypes of CDK4?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The immunogen of M00159 anti-CDK4 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-26
Question
We want to test anti-CDK4 Rabbit Monoclonal antibody M00159 on mouse right ovary for research purposes, then I may be interested in using anti-CDK4 Rabbit Monoclonal antibody M00159 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Dhar
Verified customer
Asked: 2018-10-22
Answer
The products we sell, including anti-CDK4 Rabbit Monoclonal antibody M00159, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-22
Question
We have tried in the past anti-CDK4 Rabbit Monoclonal antibody for IP on muscle a few months ago. I am using rat, and We are going to use the antibody for IF next. My question regards examining muscle as well as right ovary in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
I have checked the website and datasheets of our anti-CDK4 Rabbit Monoclonal antibody and it appears that M00159 has been tested on rat in both IP and IF. Thus M00159 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-07
Question
I am interested in using your anti-CDK4 Rabbit Monoclonal antibody for evasion of oncogene induced senescence due to defective p16ink4a binding to cdk4 studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
Thanks for your inquiry. This M00159 anti-CDK4 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-08
Question
My team were satisfied with the WB result of your anti-CDK4 Rabbit Monoclonal antibody. However we have been able to see positive staining in muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?
R. Baker
Verified customer
Asked: 2016-10-25
Answer
Based on literature, muscle does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-10-25
Question
I see that the anti-CDK4 Rabbit Monoclonal antibody M00159 works with IP, what is the protocol used to produce the result images on the product page?
N. Zhao
Verified customer
Asked: 2016-10-10
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-10-10
Question
Do you have a BSA free version of anti-CDK4 Rabbit Monoclonal antibody M00159 available?
T. Lewis
Verified customer
Asked: 2016-06-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CDK4 Rabbit Monoclonal antibody M00159 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159. Let me know if you need anything else.
C. Jha
Verified customer
Asked: 2015-12-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-14
Question
Is a blocking peptide available for product anti-CDK4 Rabbit Monoclonal antibody (M00159)?
B. Brown
Verified customer
Asked: 2015-03-12
Answer
We do provide the blocking peptide for product anti-CDK4 Rabbit Monoclonal antibody (M00159). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-03-12
