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- Table of Contents
Application | Immunofluorescence |
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Sample | wildtype and KO mutant zebrafish retina |
Primary Incubation | 1:100 |
Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
Secondary Antibody | Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution. |
Application | Immunofluorescence |
---|---|
Sample | wildtype and KO mutant zebrafish retina |
Primary Incubation | 1:100 |
Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution. |
Application | Immunofluorescence |
---|---|
Sample | wildtype and KO mutant zebrafish retina |
Primary Incubation | 1:200 |
Blocking Agent | 10% NGS, 2% BSA, 0.1% Tween in PBS |
Secondary Antibody | Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG |
"First, we received our shipment of antibodies in perfect condition. Thank you for all the work on this project. On the left, you can find pictures of 5dpf wildtype and Ush2a mutant zebrafish retina stained with DZ01481 in red. The pictures show the typical layered retinal structure, with punctate Ush2a staining at the connecting cilium (cc). In blue, the nuclei of inner and outer nuclear layers are shown (INL/ONL)."
Source: Biocompare.com
Application | ELISA |
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Starting Material | Serum |
Tips | Follow the incubation times closely |
Results Summary | Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS. |
"CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction."
Source: Biocompare.com
Application | Western Blot |
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Sample | Tumor cell lysate |
Primary Incubation | Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw |
Blocking Agent | 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) |
Secondary Incubation | Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw |
Tertiary Incubation | HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw |
Detection | Chemiluminescence: West Pico from Thermo Scientific |
Results Summary | The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃. |
"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."
Source: Biocompare.com
Application | Western Blot |
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Sample | 22RV1 cytosolic and membrane fractions |
Primary Incubation | 1:1000, overnight at 4 degree |
Blocking Agent | 5% Milk |
Secondary Incubation | 1:5000, 1 hour RT. |
Detection | ECL |
Results Summary | Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135). |
"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."
Source: Biocompare.com
Application | Test the quantity of MMP-9 released from neutrophils after challenge with bacteria |
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Starting Material | Human Neutrophils |
Protocol Overview | 1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader |
Tips | Frozen supernatants work just as well as fresh ones |
Results Summary | I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend. |
"The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones."
Source: Biocompare.com
Application | Immunohistochemistry |
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Sample | Mice kidney |
Primary Incubation | 1:100, room temp, 90 minutes |
Blocking Agent | Peroxidase blocker |
Secondary Incubation | Mouse/Rabbit Unovue, 30 minutes, room temperature |
Detection | DAB chromogen |
Results Summary | We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. |
"We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney."
Source: Biocompare.com
Application | Flow Cytometry |
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Sample | B cells |
Primary Incubation | 0.25 µl/10^6 cells 50min RT |
Blocking Agent | FBS 5% |
Secondary Incubation | F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6 |
Detection | Flow Cytometry |
Results Summary | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody. |
Source: Biocompare.com
Application | Western Blot |
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Sample | Human cell lines |
Primary Incubation | 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking |
Blocking Agent | 5% BSA in TBS |
Secondary Incubation | Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking |
Detection | Licor Odyssey IR Scanner |
Additional Notes | The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery). |
Results Summary | The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands. |
"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."
Source: Biocompare.com
Application | ELISA |
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Starting Material | Rat Plasma |
Tips | Kit suggested sample dilution is 1:50 but I used 1:25 and got results within standard range. |
Results Summary | We compared plasma FABP4 levels in pregnant and nonpregnant rats and results were consistent with reported studies. |
"There were a number of ELISA kits for human and mice FABP4 but rat-specific kits were not easily available. We tried this Kit from Boster and are happy with the kit performance. The steps were easy to follow and standers and sample values were in an acceptable range."
Source: Biocompare.com
Application | Urinary Biomarker Discovery |
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Starting Material | Urine |
Tips | Fast and good product. |
Results Summary | Orosomucoid-1 (AGP1) may have important effects on urological malignancies, both with its altering the pharmacokinetics of chemotherapeutics and its immunomodulatory and pro-angiogenicproperties. |
"I have used other ELISA kits from different brands, but this specific ELISA kit from Booster is very user-friendly and fast. Consistent test results while working with many patients."
Source: Biocompare.com
Application | Western Blot |
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Sample | Mouse Neutrophils |
Primary Incubation | 1:2000 1 hr |
Blocking Agent | 5% BSA |
Secondary Incubation | 1:3000 |
Detection | HRP |
Results Summary | Nicely compared total LTF in neutrophils to degranulated LTF. In the image below, Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20. |
"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."
Source: Biocompare.com
Application | Western Blot |
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Sample | Hello cell and mouse tissues |
Primary Incubation | 1:2000 dilution at 4oC overnight |
Blocking Agent | 5% non-fat milk |
Secondary Incubation | 1:2500 dilution at RT for 1 hour |
Detection | Fluorescence |
Results Summary | This antibody works in WB with tested samples. The signal is strong enough to get a decent image. It works for human and mouse samples. |
"This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok."
Source: Biocompare.com
Application | Western Blot |
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Sample | HEK293T |
Primary Incubation | 1:5000 for 24hours at 4degrees celsius. |
Blocking Agent | Skim milk |
Secondary Incubation | 1 hour room temperature 1:3000 |
Detection | ECL |
Results Summary | We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins. |
"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."
Source: Biocompare.com
Application | Human TSH in dried blood spots |
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Starting Material | Dried blood spots |
Tips | Use the 0 standard as a diluent, PBS works decently if not. |
Results Summary | The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range. |
"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."
Source: Biocompare.com
Application | Human progesterone quantification in dried blood spots |
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Starting Material | Dried Blood Spots |
Tips | Use the 0 standard as a diluent, don't include it on the standard curve. |
Results Summary | Our data concluded that dried blot spots can be used to quantify T3 levels in humans. The sensitivity remains relevant. Results are consistent and repeatable. |
"This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits."
Source: Biocompare.com
Application | Human Progesterone saliva levels |
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Starting Material | Saliva |
Tips | This is a competitive assay. If you use a blank well to subtract background noise, make sure to not add any reagents except stop. |
Results Summary | This product gave repeatable and reliable results. Results in the lower range (below 0.5) went OWR, consider adjusting the sensitivity. |
"The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we want to provide accurate healthcare to men and women."
Source: Biocompare.com
Application | Human T4 levels in Dried Blood Spots |
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Starting Material | Dried Blood Spots |
Tips | This is a competitive ELISA without a 0 standard or a diluent, PBS makes a reliable diluent for patient samples and standards. |
Results Summary | Results were repeatable and reliable using dried blood spots. The highest concentration standard sometimes fell OWR, but the low end stayed within reading capabilities. |
"The goal of this experiment is to quantify the levels of human T4 in dried blood spots from at-home test kits. This assay in combination with other hormone tests is aimed at providing accurate and timely healthcare to patients remotely."
Source: Biocompare.com
Application | Human Testosterone saliva levels |
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Starting Material | Saliva |
Tips | Do not use water or PBS to dilute patient sample or standards. |
Results Summary | The results from this assay were easily obtained, repeatable, and reliable. The standard curve was accurate and precise, resulting it quality result for patient samples. |
"The experiment goal was to quantify the levels of testosterone in human saliva with this booster kit designed for blood or serum samples. The aim is to provide at-home test kits for remote patients using a non-invasive collection method."
Source: Biocompare.com
Application | Immunofluorescence |
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Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
Primary Incubation | Overnight |
Blocking Agent | 3% goat serum in PBS |
Secondary Incubation | 1 hour |
Tertiary Incubation | None |
Detection | Fluorescence microscopy |
DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."
Boster bio Iba1 1:100 PFA (3-4) - cortex 12x - with scale bar
Boster bio Iba1 1:100 PFA (3-4) - cortex 25x - with scale bar
Application | Immunofluorescence |
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Sample | Mouse brain |
Primary Antibody Dilution | 1:100 |
Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of Iba1 (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.
Boster bio GFAP 1:100 PFA (7-2) - cortex 12x - with scale bar
Boster bio GFAP 1:100 PFA (7-2) - cortex 20x - with scale bar
Boster bio GFAP 1:250 12x (free-floating)
Application | Immunofluorescence |
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Sample | Mouse brain |
Primary Antibody Dilution | 1:100 |
Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of GFAP (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.
Immunohistochemistry was also performed with free-floating sections, which were exposed to the same primary antibodies diluted (1:250) in blocking buffer overnight and, subsequently, exposed to secondary antibodies diluted in blocking buffer for 1 hour, following the same procedure as the mounted sections. After incubation, sections were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse and transferred with a painting brush to a container filled with 1X PBS with 0.01% sodium azide, from which they were mounted onto slides. Slides were left to dry in the dark, after which mounting media was added to cover slip the sections. As with slide-mounted sections, these slides were kept in the dark at 4 ˚C prior to imaging.
Source: Customer Feedback Submission
--Ailin Lepletier de Oliveira
Application | IHC |
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Sample | Mouse spleen and lung |
"Very happy with this product. We were using an IL-17A from Abcam but found very high endogenous non-specific staining. This product was a much better stain in comparison. We used this antibody (1:200) on the Leica BOND Rxm autostainer, blocked with Sniper/1% BSA and it worked beautifully."
Source: Customer Feedback Submission
--Jason Tennessen
Application | IF |
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Sample | Drosophila larval fat body |
"We’ve verified the specificity of this custom antibody that Boster generated for us. The antibody "Anti-Fruit Fly Gpdh1 Antibody (DZ41223)” works well for immunofluorescence. A 1:100 dilution of this antibody stains fat body tissues of wild-type larvae but not Gpdh1 mutant. I’m happy to advertise this antibody to the fly community."
--Amin Ghabrial Pathology and Cell Biology Columbia University Principal Investigator
Excellent, submitted by Amin Ghabrial on 2021-11-29Source: Biocompare.com
"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."
Application | Immunofluorescence |
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Sample | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Drosophila larvae |
Primary Incubation | 1:100 at 4 degrees overnight |
Blocking Agent | 4% horse serum |
Secondary Incubation | 1:1000 anti-Rb-A488 |
Tertiary Incubation | N/A |
Detection | IF |
Results Summary | Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear. |
--Nikhil Kumar
Excellent, submitted by Nikhil Kumar on 2021-11-18Source: Customer Feedback Submission
Application | IHC |
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Sample | Uveal melanoma |
"I am using Aqueous mounting media for IHC in Uveal Melanoma sections. The substrate is AEC for peroxidase enzyme, which produces a red end product that is soluble in alcohol."
--Yair Fisher, GliXogen Therapeutics, CSO
Excellent, submitted by Yair Fisher on 2021-11-02Source: Customer Feedback Submission
Application | Flow Cytometry |
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Sample | C3H10T1/2 mouse cell line |
"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."
--Lakshmi, Biotech
Excellent, submitted by Lakshmi on 2021-08-23Source: Google.com
Applications | Immunohistochemistry |
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Sample | Kidney |
Primary antibody dilution | F4/80 Primary antibody / 1:500 |
"We have been struggling to get a good signal for macrophages in diabetic kidney. However, this kit helped us get a very good signal in the diabetic kidney compared to the controls. Will use this kit for all the chromogenic stainings planned for my study. Very simple to use with promising results!"
--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine
Excellent, submitted by Yuzuru Imai on 2021-04-29Application | Western blot |
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Sample | Drosophila melanogaster (head and thorax) |
Detection | Immobilon Forte Western HRP substrate |
"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."
--Jingli Cao, Assistant Professor, Weill Cornell Medical College
Excellent, submitted by Jingli Cao on 2021-04-13Application | Immunofluorescence |
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Sample | Zebrafish heart |
Detection | Confocal Microscopy |
"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."
Application | Immunohistochemistry (Paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Lung |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher
Excellent, submitted by Xiangrong Geng on 2020-07-30Source: Biocompare.com
Application | Western Blot |
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Sample | HEK293T |
Detection | ECL |
"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."
--Daisy Dai University of Miami Surgery Lab Director / Manager
Excellent, submitted by Daisy Dai on 2020-07-30Source: Biocompare.com
Application | ELISA |
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Starting Material | Crispr MEK and STAT3 KO CAFs |
Tips | This kit doesn't provide PC |
"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."
--John Ong, University of Cambridge Engineering Graduate Student
Excellent, submitted by John Ong on 2020-07-30Source: Biocompare.com
Application | Testing laminin concentration in hydrogel |
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Starting Material | Hydrogel |
Tips | Supernatant used for ELISA |
"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."
Application | Immunohistochemistry (paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Mouse Brain |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37 °C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2 ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin embedded) |
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Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
Sample | Prostate cancer |
Fixative | Fixed with 4% paraformaldehyde |
Primary Incubation | 4°C overnight |
Primary Incubation diluent | 5% BSA in TBS |
Primary Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Dilution | The kit was ready to use, no dilution needed |
Secondary Incubation | at 37°C for 30 min |
The antibody worked perfectly, Great sensitivity
Application | Immunohistochemistry (paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Mouse Liver |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | 4°C overnight |
Application | Immunohistochemistry (Paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Oesophagus |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Tonsil |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 0.5 ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunocytochemistry |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Colorectal tissue |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin embedded) |
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Blocking step | 5% BSA as blocking agent for 30 min at 37°C |
Sample | Placenta |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin-embedded) |
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Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Mouse skin |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist
Excellent, submitted by Svetlana Dzitoyeva on 2020-01-10Source: Biocompare.com
Application | Western Blot |
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Sample | Insect, D. melanogaster |
Detection | ECL |
"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."
--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor
Excellent, submitted by Hanjo Hellmann on 2020-01-10Source: Biocompare.com
Application | Western Blot |
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Sample | Recombinant protein expressed in and purified from E. coli |
Detection | Chemiluminescence |
"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."
--Ramaz Geguchadze, University of New England, Biomedical Sciences, Research Scientist
Excellent, submitted by Ramaz Geguchadze on 2020-01-10Source: Biocompare.com
Application | Western Blot |
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Sample | Dorsal root ganglia (neuronal cells) |
Detection | CyDye |
"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."
--Dinesh Joshi, University of Wisconsin Madison, Research Scientist
Excellent, submitted by Dinesh Joshi on 2019-12-06Source: Biocompare.com
Application | Immunohistochemistry |
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Sample | Mice Cerebellum |
Detection | Fluorescence |
"I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P."
--David Nelson, University of Wisconsin, Research Scientist
Excellent, submitted by David Nelson on 2019-12-06Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Mouse plasma (with EDTA as anticoagulant) |
Detection | -- |
"Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays. 10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad. Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% - but consistent with the product literature. I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37°C) because I didn't want to deal with going back and forth into the room that houses our 37°C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image, Columns A and B are the standard curve." (Customer’s Tip: "100-fold dilution of mouse plasma resulted in all data falling on standard curve.")
--Abdullah Mamun, University of Texas Health Science Center at Houston, Neurology, Postdoctoral Researcher
Excellent, submitted by Abdullah Mamun on 2019-12-06Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Serum |
Detection | -- |
"Good sensitivity. I have found detectable levels of CD200. Overall, it is a very good kit for CD200 detection." (Customer’s Tip: "I have used neat samples.")
--Jakub Famulski, University of Kentucky, Biology, Principal Investigator
Excellent, submitted by Jakub Famulski on 2019-12-06Source: Biocompare.com
Application | Immunofluorescence |
---|---|
Sample | Zebrafish embryos, 5 days old |
Detection | Confocal Microscopy |
"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"
--Jakub Famulski, University of Kentucky, Biology, Principal Investigator
Excellent, submitted by Jakub Famulski on 2019-12-06Source: Biocompare.com
Application | Immunofluorescence |
---|---|
Sample | Zebrafish embryos 5dpf |
Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
--Jakub Famulski, University of Kentucky, Biology, Principal Investigator
Excellent, submitted by Jakub Famulski on 2019-12-06Source: Biocompare.com
Application | Immunofluorescence |
---|---|
Sample | Zebrafish embryos 24hpf |
Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."
--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician
Excellent, submitted by Harrison Esterly on 2019-12-06Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Mouse serum |
Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")
--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician
Excellent, submitted by Harrison Esterly on 2019-12-06Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Mouse serum |
Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Liver |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C Overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Rectum |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 0.5ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | 4°C overnight |
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Rat Brain Tissue |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | 4°C overnight |
Application | Immunohistochemistry (Paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Ovary |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | 4°C overnight |
Application | Immunohistochemistry (Paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human tonsil |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | 4°C overnight |
Source: Biocompare.com
---P DPN, Johns Hopkins University, Pathology, Postdoctoral Researcher
Application | Immunohistochemistry |
---|---|
Sample | Mice kidney |
Detection | DAB chromogen |
"We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it."
Application | Immunofluorescence (IF) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human rectal |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC
kit from Boster Bio, (SA1022 |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE
Excellent, submitted by Junhui Wang on 2019-05-24Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Brain Tissue Lysate |
Detection | -- |
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
--Mostafa Gabr, Duke University Hospital, Neurosurgery, Postdoctoral Researcher
Excellent, submitted by Mostafa Gabr on 2019-05-21Source: Biocompare.com
Application | Immunohistochemistry |
---|---|
Sample | Rat spinal cord |
Detection | Fluorescent microscope |
"I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously. Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord. Excellent, reliable product and easy to use."
--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Sanjay Arora on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Cell lysate and culture medium |
Detection | -- |
"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")
--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Sanjay Arora on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Serum, tissue homogenate |
Detection | -- |
"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Divya Sharma on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Cell culture media |
Detection | -- |
"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Divya Sharma on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Cell culture media, mouse serum |
Detection | -- |
"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Divya Sharma on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Centrifuged cell culture media supernatant |
Detection | -- |
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")
--Xiaobing Cui, University of Georgia, Physiology & Pharmacology, Research Scientist
Excellent, submitted by Xiaobing Cui on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Serum and supernatant |
Detection | -- |
"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
Excellent, submitted by Divya Sharma on 2019-05-21Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Cell culture media |
Detection | -- |
"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Placenta |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 0.5ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
Application | Immunohistochemistry (paraffin-embedded ) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human colon |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37 °C for 30 minutes |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 2ug/ml |
Secondary Antibody | SABC
kit from Boster Bio, (SA1022 |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
--Preethi Vijayaraj, UCLA, Pediatrics, Postdoctoral Fellow
Excellent, submitted by Preethi Vijayaraj on 2019-02-15Source: Biocompare.com
Application | ELISA |
---|---|
Sample | Cell culture supernatant |
Detection | -- |
"Our research focuses on in vitro modeling of organ fibrosis and drug discovery. The protocol for this product was easy to perform. However, there was some amount of variability with the replicates. Was effective in detecting A2M levels at pg levels. Reasonable sensitivity and reproducibility. Easy to use." (Customer’s Tip: "Follow protocol to the T.")
--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist
Excellent, submitted by Saikat Chakraborty on 2019-02-15Source: Biocompare.com
Application | Western Blot |
---|---|
Sample | Human Tissue |
Detection | Fluorescent based |
"We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 antibody worked perfectly in detection in an Alzheimer's tissue sample. No extra bands. I recommend."
--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist
Excellent, submitted by Saikat Chakraborty on 2019-02-15Source: Biocompare.com
Application | Western Blot |
---|---|
Sample | Human Tissue |
Detection | Fluorescent based |
"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."
--Swati Naphade, Aging, Buck Institute, Postdoctoral Fellow
Excellent, submitted by Swati Naphade on 2019-02-15Source: Biocompare.com
Application | Western Blot |
---|---|
Sample | Human NSC cell lysates |
Detection | ECL chemiluminescence |
"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Bowel |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 min |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
This antibody works perfectly well!
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Prostate |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4 °C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1 ug/ml |
Secondary Antibody | Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) |
Secondary Ab Dilution | Use at an assay dependent concentration |
Secondary Ab Incubation | at 37°C for 30 min |
--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate
Excellent, submitted by Dhruva Bhattacharya on 2017-12-22Source: Biocompare.com
Application | ELISA |
---|---|
Sample | "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells |
Detection | -- |
"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."
--Hua Jiang, Pediatrics, University of Colorado Denver, Research Associate
Excellent, submitted by Hua Jiang on 2017-11-29Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Human liver, rat liver, mouse liver, mouse kidney, and mouse brain |
Detection | Typhoon 940 |
"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."
--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University
Excellent, submitted by Saikat Chakraborty on 2017-11-29Source: Customer Feedback Submission
Applications | Western Blot |
---|---|
Sample | Mouse brain lysate |
Detection | Licor Imaging and ImageJ |
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
--Peter J. Thompson, UCSF Diabetes Center, Postdoctoral Scholar
Excellent, submitted by Peter J. Thompson on 2017-08-23Source: Customer Feedback Submission
Applications | IHC-Paraffin, IHC-cryo |
---|---|
Sample | NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded |
Detection | Zeiss Apotome widefield fluorescence microscope |
"CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks."
--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor
Excellent, submitted by Chad Borges on 2017-08-21Source: Customer Feedback Submission
Applications | Picomole scale immunoprecipitation |
---|---|
Sample | Rat EDTA plasma |
Detection | Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF) |
"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."
--Akshay Jain, University of Missouri, Kansas City, Pharmaceutical Sciences, Research Assistant
Excellent, submitted by Akshay Jain on 2017-08-21Source: Biocompare.com
Applications | ELISA (Inflammatory cytokine assay from plasma) |
---|---|
Sample | Rat plasma |
Detection | -- |
"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")
--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA
Excellent, submitted by Stacy Wilson on 2017-08-21Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | MDA-MB-468 and HEK293E cell lysates |
Detection | SuperSignal West Pico Plus with a BioRad imager |
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist
Excellent, submitted by Huiyuan Tang on 2017-08-21Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA |
Detection | -- |
"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")
--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow
Excellent, submitted by Mahesh Raundhal on 2017-08-10Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Human ovarian cancer ascites |
Detection | -- |
"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")
--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR
Excellent, submitted by Chi Li, University of Louisville on 2016-10-20Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Whole cell extract of pancreatic tumor cell MIA PaACa2 |
Detection | Enhanced chemiluminescence |
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT
Excellent, submitted by Ruby C on 2016-09-13Source: Biocompare.com
Applications | Immunohistochemistry |
---|---|
Sample | Mouse astrocytes |
Detection | Confocal/Fluorescent Scope |
"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."
--Milos Lazic, JECURE THERAPEUTICS, SCIENTIST II
Excellent, submitted by Milos Lazic on 2016-08-27Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | protein concentrations in cell culture media |
Detection | -- |
"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."
--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST
Excellent, submitted by Charles Stopford on 2016-08-24Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Primary keratinocytes |
Detection | -- |
"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."
--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT
Excellent, submitted by Chun-Chih Tseng on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Human breast cancer cell line |
Detection | Bio-Rad imager |
"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."
--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS
Excellent, submitted by Christina Klinger on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Mouse lung lysates |
Detection | SuperSignal West Femto Maximum Sensitivity Substrate |
"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)
Excellent, submitted by Unattributed Review* on 2016-08-24Source: Biocompare.com
Applications | IL-1RA detection in rat serum |
---|---|
Sample | Rat serum |
Detection | -- |
"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."
--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST
Excellent, submitted by Tabitha Doci on 2016-08-24Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Purified mouse prolactin and plasma |
Detection | TMB Substrate |
"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."
--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE
Excellent, submitted by Sona Balasubramaniam on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | MDCK cell lysate |
Detection | ECL |
"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."
--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT
Excellent, submitted by Yongzhen Guo on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
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Sample | Rat endothelial cells |
Detection | Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096) |
"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")
Excellent, submitted by Unattributed Review* on 2016-08-24Source: Biocompare.com
Applications | Immunohistochemistry |
---|---|
Sample | Wistar rat brain |
Detection | Fluorescence |
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."
--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN
Excellent, submitted by R.P. on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Cell lysate |
Detection | Enhanced chemiluminescence |
"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."
--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER
Excellent, submitted by Gang Ye on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
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Sample | Human cell lines |
Detection | ECL |
"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."
--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
Excellent, submitted by Elena Deryugina on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Tumor cell lysate and recombinant protein |
Detection | SuperSignal West Pico Chemiluminescent Substrate |
"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."
--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
Excellent, submitted by Elena Deryugina on 2016-08-24Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane |
Detection | SuperSignal West Pico Chemiluminescent Substrate |
"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."
--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC
Excellent, submitted by Maria Teresa Dell'Anno on 2016-08-24Source: Biocompare.com
Applications | Immunohistochemistry |
---|---|
Sample | Spinal cord |
Detection | Confocal microscope |
"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."
--Lu Yang, UCSD, CMMW, GRADUATE STUDENT
Excellent, submitted by Lu Yang on 2016-02-17Source: Biocompare.com
Applications | Protein level screen |
---|---|
Sample | Cell culture media from MCF7 cells |
Detection | -- |
"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."
--Michael Conlin, GENETICS AND MOLECULAR BIOLOGY, UNC CHAPEL HILL, GRADUATE STUDENT
Excellent, submitted by Michael Conlin on 2016-02-17Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | MEFs |
Detection | LICOR Odyssey |
"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."
--Aijun Qiao, CANCER CENTER, AUGUSTA UNIVERSITY, POSTDOCTORAL FELLOWS
Excellent, submitted by Aijun Qiao on 2016-01-21Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Subcutaneous fat and brown fat tissue |
Detection | Western Blot |
"We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis. Although there are some non-specific bands, we still can get the strong and clear bands that we want to. The WB band is strong even when you use high dilution concentration. Overall this antibody is good to check UCP1 levels using WB technology."
--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT
Excellent, submitted by Hannah Oakes on 2016-01-21Source: Biocompare.com
Applications | Detecting GDNF |
---|---|
Sample | Brain Tissue (Hippocampus) |
Detection | -- |
"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."
--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT
Excellent, submitted by Prakash A on 2016-01-19Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Jurkat Lysates |
Detection | ECL solution |
"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."
--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT
Excellent, submitted by Shubhankar Nath on 2016-01-19Source: Biocompare.com
Applications | Western Blot |
---|---|
Sample | Jurkat cell lysate |
Detection | Chemiluminescence |
"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."
--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE
Excellent, submitted by Jason Somarelli on 2016-01-05Source: Biocompare.com
Applications | Detection of secreted IL-2 in media of Jurkat T cells |
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Sample | Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep) |
Detection | -- |
"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."
--J L, UIC, DENTISTRY, SCIENTIST
Excellent, submitted by J L on 2016-01-05Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Skin tissue homogenates |
Detection | -- |
"This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased at the proliferation/remolding stage. Easy to use and less expensive. Highly recommended."
--Tho Pham, UCONN, NUTRITIONAL SCIENCES, GA
Excellent, submitted by Tho Pham on 2015-12-29Source: Biocompare.com
Applications | ELISA |
---|---|
Sample | Cell culture supernatant |
Detection | -- |
"This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon."