Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | PB9221 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IKK beta/IKBKB Antibody Picoband®
SKU/Catalog Number
PB9221
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKK beta/IKBKB Antibody Picoband® catalog # PB9221. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK beta/IKBKB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9221)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9221 is reactive to IKBKB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
86564 MW
Background of IKK beta
IKBKB (Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene. This gene is mapped to chromosome 8p12-p11 by FISH. It is found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. The activity of various antiinflammatory agents on the IKK complex has been texted, it has been found that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9221 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: HEPG2 Whole Cell, COLO320 Whole Cell, M231 Whole Cell, HT1080 Whole Cell
IHC: Human Lung Cancer Tissue
ICC/IF: A431 cell
FCM: 293T cell
Validation Images & Assay Conditions
Click image to see more details
Anti-IKK beta antibody, PB9221, Western blotting
All lanes: Anti IKK beta (PB9221) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: M231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
Click image to see more details
Anti-IKK beta antibody, PB9221,IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-IKK beta antibody, PB9221, Western blotting
All lanes: Anti IKK beta (PB9221) at 0.5ug/ml
WB: Recombinant Human IKK beta Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
Click image to see more details
Figure 4. IF analysis of IKK beta using anti-IKK beta antibody (PB9221).
IKK beta was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IKK beta Antibody (PB9221) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of 293T cells using anti-IKK beta antibody (PB9221).
Overlay histogram showing 293T cells stained with PB9221 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (PB9221,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For IKBKB (Source: Uniprot.org, NCBI)
Gene Name
IKBKB
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
Weight
86564 MW
Superfamily
protein kinase superfamily
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;2.7.11.10;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;IKBKB;IKKB; IKBKB IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB inhibitor of nuclear factor kappa B kinase subunit beta inhibitor of nuclear factor kappa-B kinase subunit beta|I-kappa-B kinase 2|I-kappa-B-kinase beta|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta|nuclear factor NF-kappa-B inhibitor kinase beta|serine/threonine protein kinase IKBKB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKB, check out the IKBKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK beta/IKBKB Antibody Picoband® (PB9221)
Hello CJ!
No publications found for PB9221
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IKK beta/IKBKB Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IKK beta/IKBKB Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-IKK beta/IKBKB Antibody Picoband®
Question
We are currently using anti-IKK beta/IKBKB antibody PB9221 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The anti-IKK beta/IKBKB antibody (PB9221) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-22
