Buffer Recipes

Boster Bio Common Buffers

Learn about buffer recipes and find pre-made easy to use buffers packets for protein extraction, washing, and many more applications. Our buffers have been cited in over 6,000 publications.

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Citrate Buffer Powder

74 References 1 Q&As

2 images

$3
Cat # AR0024
1 pack

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The Buffers in science

Buffers are essential for several steps in many protocols to generate publishable data. Boster provides a wide range of general purpose buffers, extraction or lysis buffers as well as buffers specific for western blot, immunohistochemistry, ELISA, and other applications. Learn about what buffers can be used for different applications and see our buffer formulations for the most commonly used buffers.

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    Buffer Selection Table

    Check out which buffers can be used for different applications

    Buffer SKU Application Buffer Notes Buffer Recipe
    Citrate Buffer Powder AR0024 IHC For 2000 mL (pH 6): 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
    RIPA Lysis Buffer
    • AR0105-100
    • AR0102-100
    WB, ELISA, IF, IP
    • Extraction/lysis buffer
    • Complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells
    • Effectively extracts cytoplasmic, nuclear, and membrane proteins
    For 100mL: 50mM Tris•HCl pH 7.6, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS
    SDS-PAGE Protein Loading Buffer 2X (Reducing) AR0131-20 WB
    • Most commonly used sample buffer for SDS-PAGE of denatured proteins in the Laemmli SDS-PAGE system
    For 20mL: 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromophenol blue and 0.1 M Tris HCl, pH 6.8
    SDS-PAGE Protein Loading Buffer 5X (Reducing)
    • AR1112
    • AR1112-20
    WB
    • Most commonly used sample buffer for SDS-PAGE of denatured proteins in the Laemmli SDS-PAGE system
    10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, pH 6.8
    Antibody Diluent
    • AR1016-2
    • AR1017
    WB, IHC, ELISA
    • General purpose buffer
    • Used to prepare primary antibodies (polyclonal & monoclonal)
    • Helps reduce background in IHC staining process
    4% Paraformaldehyde (PFA) Solution in PBS AR1068 IHC
    • Ready-to-use solution for sample preparation and fixing cells for IHC
    Sample Diluent Buffer AR1106-1 ELISA
    • Used to dilute samples
    ABC Diluent Buffer AR1106-3 ELISA
    • For HRP complex dilution
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