Click image to see more details
Product Info Summary
| SKU: | M00003 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
SKU/Catalog Number
M00003
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-mTOR/Tor Rabbit Monoclonal Antibody catalog # M00003. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mTOR/Tor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00003)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CBD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human mTOR
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00003 is reactive to MTOR in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
289 kDa
Calculated molecular weight
288892 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00003 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
FC 1:20
Positive Control
WB: human 293T whole cell, human Hela whole cell, human K562 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MTOR using anti-MTOR antibody (M00003).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified monoclonal antibody (Catalog # M00003) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289 kDa. The expected band size for MTOR is at 289 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat liver, using mTOR Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MTOR (Source: Uniprot.org, NCBI)
Gene Name
MTOR
Full Name
Serine/threonine-protein kinase mTOR
Weight
288892 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTOR, check out the MTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-mTOR/Tor Rabbit Monoclonal Antibody (M00003)
Hello CJ!
M00003 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
Species: Rat
M00003 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
M00003 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Correlation between autophagy of osteoblasts and oxidative stress of osteoporosis rats
Rapamycin provides anti%u2011epileptogenic effect in a rat model of post%u2011traumatic epilepsy via deactivation of mTOR signaling pathway
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-mTOR/Tor Rabbit Monoclonal Antibody
Question
Would anti-mTOR/Tor Rabbit Monoclonal antibody M00003 work on pig IHC with testis?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Our lab technicians have not tested anti-mTOR/Tor Rabbit Monoclonal antibody M00003 on pig. You can run a BLAST between pig and the immunogen sequence of anti-mTOR/Tor Rabbit Monoclonal antibody M00003 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
I see that the anti-mTOR/Tor Rabbit Monoclonal antibody M00003 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-22
Question
Is a blocking peptide available for product anti-mTOR/Tor Rabbit Monoclonal antibody (M00003)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We do provide the blocking peptide for product anti-mTOR/Tor Rabbit Monoclonal antibody (M00003). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-23
Question
Do you have a BSA free version of anti-mTOR/Tor Rabbit Monoclonal antibody M00003 available?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR/Tor Rabbit Monoclonal antibody M00003 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-mTOR/Tor Rabbit Monoclonal antibody for WB for mouse cerebellum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cerebellum identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, M00003 anti-mTOR/Tor Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We have seen staining in rat cervix carcinoma. What should we do? Is anti-mTOR/Tor Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
From what I have seen in literature cervix carcinoma does express MTOR. From what I have seen in Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-09-16
Question
Would anti-mTOR/Tor Rabbit Monoclonal antibody M00003 work for WB with cerebellum?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
According to the expression profile of cerebellum, MTOR is highly expressed in cerebellum. So, it is likely that anti-mTOR/Tor Rabbit Monoclonal antibody M00003 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2019-07-26
Question
you antibody using your anti-mTOR/Tor Rabbit Monoclonal antibody for pip3 activates akt signaling studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We appreciate your inquiry. This M00003 anti-mTOR/Tor Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-18
Question
Does M00003 anti-mTOR/Tor Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
As indicated on the product datasheet, M00003 anti-mTOR/Tor Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-11
Question
Our team were satisfied with the WB result of your anti-mTOR/Tor Rabbit Monoclonal antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Based on literature, liver does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-04
Question
I am looking for to test anti-mTOR/Tor Rabbit Monoclonal antibody M00003 on mouse cerebellum for research purposes, then I may be interested in using anti-mTOR/Tor Rabbit Monoclonal antibody M00003 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Brown
Verified customer
Asked: 2019-05-24
Answer
The products we sell, including anti-mTOR/Tor Rabbit Monoclonal antibody M00003, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-24
Question
Is this M00003 anti-mTOR/Tor Rabbit Monoclonal antibody reactive to the isotypes of MTOR?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
The immunogen of M00003 anti-mTOR/Tor Rabbit Monoclonal antibody is A synthesized peptide derived from human mTOR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-15
Question
We are currently using anti-mTOR/Tor Rabbit Monoclonal antibody M00003 for rat tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
V. Baker
Verified customer
Asked: 2018-04-12
Answer
The anti-mTOR/Tor Rabbit Monoclonal antibody (M00003) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-mTOR/Tor Rabbit Monoclonal antibody M00003. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-11
Question
Our lab used your anti-mTOR/Tor Rabbit Monoclonal antibody for IF on testis in the past. I am using mouse, and We want to use the antibody for ICC next. Our lab want to know about examining testis as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ICC?
A. Huang
Verified customer
Asked: 2017-01-23
Answer
I have checked the website and datasheets of our anti-mTOR/Tor Rabbit Monoclonal antibody and I see that M00003 has been validated on mouse in both IF and ICC. Thus M00003 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-01-23
Question
Please see the WB image, lot number and protocol we used for cerebellum using anti-mTOR/Tor Rabbit Monoclonal antibody M00003. Please let me know if you require anything else.
L. Collins
Verified customer
Asked: 2016-09-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-15
Question
Can you help my question with product M00003, anti-mTOR/Tor Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Mangal
Verified customer
Asked: 2013-05-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00003 anti-mTOR/Tor Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-05-01
