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We will stain your slides and you will get more done. Boster Bio offers easy-to-work-with histopathology services with a fast turnaround time.
Our IHC experts have experience in
*Multiplex staining, digital scanning, and image analysis are available.
Choose the option that best describes you.
Because we are an antibody manufacturer with pathology focus
Since 1993, Boster has been manufacturing antibodies and reagents for pathology/IHC. Our founder came from a medical histology background and IHC has been in the company's DNA from day 1. With hundreds of IHC experiments performed each week and after 3 decades of continuous improvement, Boster's technical team has mastered the art of IHC. Till end of 2019, there were more than 5,000 articles published using IHC results obtained from Boster antibodies (source: Google Scholar, Bioz and CiteAb). With our history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings with our IHC service.
"The rat brains were beautifully sectioned."
"Boster Bio performed a very thorough IHC validation. Ken was professional and accommodating throughout the project. We will come back to do some more work with them. "
"Provided superior quality IHC images."
"Their service is fast and the cost is reasonable."
PhD-level support
great communication
Experience
since 1993
30,000+
publications
Proprietary
technologies
40,000+
antibodies made
Which services are you considering?
We optimize the antibody to find the best working dilution ratio. Then, we stain tissues and cells of interest on an automated system.
If your samples require preparation, our immunohistochemistry services include the processing, embedding, and sectioning of samples. Services are available for the following types of samples:
For a new antibody (i.e. antibody that has not been validated with IHC), we start off with the initial optimization service to determine the best epitope retrieval method and primary antibody incubation concentration. Having developed many different IHC assays for customers, we found that the following two strategies work quite well for most cases:
Plan | Standard Price | Sample | Epitope Retrieval | Antibody Concentration |
---|---|---|---|---|
1 | Inquire | CPA or TMA | Acidic ER: Citrate Basic ER: EDTA or Tris/EDTA |
5 concentration titrations (2-log): • Mouse antibody: 5, 2.5, 1, 0.25, 0.05 μg/ml • Rabbit antibody: 2, 1, 0.4, 0.1, 0.02 μg/ml |
2 | Inquire | CPA or TMA | Acidic ER: Citrate Basic ER: EDTA or Tris/EDTA |
3 concentration titrations: 2X, 1X, 0.5X of recommended concentration using an IHC tested antibody |
If required, we will perform another round of optimization using Plan 2, starting with the selected concentration from Phase 1
*IF/ICC: We adopt similar principles in designing immunofluorescence (IF) and
immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.
We will then validate the antibody on sample sets using the conditions set in Phase 1. We can carry out the study automated on Leica Bond automated system or manually if requested. We offer cell pellet arrays (CPA) and tissue microarrays (TMA) that consist of different expression levels of the target of interest as the validation sample. To get the results in the most cost-effective way, we recommend customers to test on these arrays, because they can get the answer they want from a single slide (per testing condition).
Finding suitable samples is key to validating the specificity and sensitivity of the assay. We have in-house multi-human tissue arrays, both normal and tumor, for that purpose. We have a variety of samples for IHC development, including FFPE or frozen cell pellet arrays (CPA) and tissue microarrays (TMA) from human, mouse, rat, and etc. Our Special FFPE Cell Pellets Technique enables us to make cell smear slides with uniquely high homogeneity. Anecdotal feedback from our customers say it is the smoothest in the industry.
We can also accept sample slides provided by the customers.
For using our samples instead of providing your own, please contact us for sample availability and pricing. We can also procure special samples (e.g. disease specific) for you as part of our procurement concierge service.
*IF/ICC: We adopt similar principles in designing immunofluorescence (IF) and
immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.
Scanning: We perform whole slide digital scanning using a Leica scanner. You can choose our routine 40X single layer bright field imaging. Multiple focal plane scanning (40X) is also available, which will yield one compiled image with guaranteed focus in every field.
Analysis: Based on your project needs, our team can offer the following analyses:
Take your immunohistochemistry to the next level with multiplexing capabilities! Our innovative multiplex IHC solutions empower you to efficiently analyze multiple protein targets in a single experiment, saving time and resources while maximizing data output.
Tell us about your project and its requirements, so we can determine how to best serve you. Start your inquiry today.
After we receive the samples, Boster will perform whichever IHC service(s) you requested.
You will receive the IHC images. For tissue processing service, we will ship the processed tissues to you.
Below are some of the thousands of IHC images we've developed internally and for our customers