Click image to see more details
Product Info Summary
| SKU: | P00003-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00003-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody catalog # P00003-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00003-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IEG-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-mTOR (S2481)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00003-1 is reactive to MTOR in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
53 kDa
Calculated molecular weight
288892 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00003-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: 293 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MTOR (Source: Uniprot.org, NCBI)
Gene Name
MTOR
Full Name
Serine/threonine-protein kinase mTOR
Weight
288892 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTOR, check out the MTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (P00003-1)
Hello CJ!
P00003-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xia Y,Zhang G,Han C,Ma K,Guo X,Wan F,Kou L,Yin S,Liu L,Huang J,Xiong N,Wang T.Microglia as modulators of exosomal alpha-synuclein transmission.Cell Death Dis.2019 Feb 20;10(3):174.doi:10.1038/s41419-019-1404-9.PMID:30787269;PMCID:PMC6382842.
Species: Human,Mouse
P00003-1 usage in article: APP:WB, SAMPLE:BV2 CELL, DILUTION: 1:400
Zhang X,Zhang L,Chen Z,Li S,Che B, Wang N,Chen J,Xu C,Wei C.Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.Int J Mol Med.2021 Mar;47(3):27.doi:10.3892/ijmm.2021.4860.Epub 2021 Feb 4.PMID:33537831.
Species: Rat
P00003-1 usage in article: APP:WB, SAMPLE:RENAL TISSUE AND PODOCYTES, DILUTION:1:600
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
Correlation between autophagy of osteoblasts and oxidative stress of osteoporosis rats
Rapamycin provides anti%u2011epileptogenic effect in a rat model of post%u2011traumatic epilepsy via deactivation of mTOR signaling pathway
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Question
My boss were content with the WB result of your anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody. However we have observed positive staining in b-cell endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?
R. Li
Verified customer
Asked: 2020-04-09
Answer
According to literature, b-cell does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody P00003-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody (P00003-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
We have been able to see staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
According to literature cervix carcinoma does express MTOR. According to Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-07-09
Question
A customer would like to know if P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) can be used on porcine. Could you help confirm with Abways?
Verified Customer
Verified customer
Asked: 2013-03-05
Answer
P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) haven't been woked on porcine sample. Abways is uncertain if they are suitable for porcine sample. The immnogen sequences of P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) are CPESIH-pS-FIGDGL and CSRTRTD-pS-YSAG respectively.
Boster Scientific Support
Answered: 2013-03-05
