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Lean, mean protein production machine

RECOMBINANT PROTEIN EXPRESSION SERVICE

Boster Bio offers one of the most efficient recombinant protein services. We perform highly successful protein expressions all year round.

  • 3000+ Expressions/year
  • 20 years of Protein Production
  • 98% Expression Success Rate

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Get a free quote

Our Specialty: Recombinant Protein Expression Services for Antigens/Immunizations

We can do simple E. Coli protein expressions with what seems to be the lowest cost on the market. However, we also offer a comprehensive suite of proteins expression services using various expression systems: E. Coli, baculovirus, insect cells, yeast, membrane proteins, and mammalian cells. Downstream services are available as well— offinity purification, protein characterization, desalting, lyophilization, packaging, and product information labeling.

  • Fast turnaround

    • 2-week rapid expression

  • Solubility expert

    • Proprietary technology for maximizing solubility

  • Cost efficiency

    • Economy of scale lowers cost

  • Comprehensive Protein Expression Services

    • Purification, protein characterization, desalting, tag cleaving, tag removal, the whole shebang

 
lean-mean-protein-expression-machine

Our codon optimization

We developed our own expression optimization algorithm. Compared to similar algorithms, ours emphasizes more on using optimal codons, which may cause difficulties in gene synthesis (shifting risks upstream). This results in better expression success and solubility than if you were to use conventional plasmid/gene optimization software.

We promise You a concierge sourcing experience

Scholars serving scholars

It is our project concierges' mission to make your project experience as smooth and memorable as possible. They are subject matter experts who are easily accessible around the clock, always happy to help you solve problems, make recommendations and sort through options.

Book a meeting with your project concierge

Featured Protein Expression Service Packages

We do everything from recombinant expressions to large-scale productions. We have all your needs covered.

e. coli
Pilot expression services



Our signature service package covers most simple protein expression and purification needs with super high efficiency.

Recombinant
Antibody production



For scaling up the production of recombinant proteins. For more details see our rabbit monoclonal antibody service.

Antigen
design & production



Looking to design and produce an antigen for drug discovery? Seek no further and talk to Boster Bio today.

Protein production in mammalian cells


Need complex proteins with PTM? We got you covered from in silico design to scale up protein production services.

Available Protein Expression Systems

We use various protein expression and purification systems to address your requirements. Here's how they compare:

Coli Expression


 
Cost ++
Speed +++
Yield +++
PTMs  
Culture Conditions +
Scale-up +++

Yeast Expression


 
Cost +
Speed +++
Yield +++
PTMs +
Culture Conditions +
Scale-up +++

Insect cells & baculovirus

 
Cost ++
Speed ++
Yield ++
PTMs ++
Culture Conditions ++
Scale-up ++

Mammalian expression

 
Cost +++
Speed +
Yield +
PTMs +++
Culture Conditions +++
Scale-up +
Expression System Advantages Challenges Common Uses
 

E. Coli
  • Low cost
  • Simple culture conditions
  • Rapid expression
  • Easy scale-up
  • Parallel cloning
  • Lack of post-translational modifications
  • Protein solubility
  • Inclusion body formation
  • Inefficient expression of higher MW proteins
  • Prokaryotic & bacterial protein
  • Functional assays
  • Structural analysis
  • Antibody/Antigen proteins
 

Yeast
  • Low cost
  • Industrial-scale yields via fermentation
  • Post-translational modifications possible
  • Rapid expression
  • Simple media
  • Improper glycosylation
  • High yields require fermentation
  • Culture conditions for optimal growth
  • High mannose modification
  • Low MW proteins
  • Enzyme studies
  • Cytokine studies
  • Gram per liter fermentation yields
 

Insect / Baculovirus
  • Post-translational modifications possible
  • Protein processing similar to mammals
  • Protein solubility
  • Static or suspension cultures
  • Suitable for toxic proteins
  • Lack of partial glycosylation
  • More complex culture conditions
  • Baculovirus vector production time-consuming
  • Longer production time
  • Transmembrane proteins
  • Secretory proteins
  • Intracellular/cytoplasmic protein
  • Toxic protein analysis
 

Mammalian
  • Complex post-translational modifications
  • High-level protein processing
  • Robust bioactive protein expression
  • Low endotoxin levels
  • Rigorous culture conditions
  • Higher cost
  • Lower yields
  • Long production time
  • Antibody fragments
  • Complex protein expression
  • Secretory proteins
  • Extracellular transmembrane proteins

Other Antibody Tests & Services


Recombinant Protein Expression Services Process

 

  • Free consultation (optional)

    We learn about your project and design the best strategy to serve your unique requirements. Please enter a valid email address, and we will give you a free quote.

  • In silico prediction (optional)

    We take your requirements and run them through our AI-powered algorithm, which learns from our past expressions and predict the best expression conditions for your protein sequence.

  • Pilot expression: gene synthesis

    Our partner makes the DNA. Takes 1-2 weeks typically.

  • Pilot Expression: small scale (Pilot) expression

    We express your target protein on a small scale to optimize expression conditions. Our custom protein expression services have proprietary techniques to improve solubility. The purified proteins are sent to you as deliverable. Our expression system usually takes 2 weeks for E. coli, and longer for more complex expression systems, and for some hard to express proteins it requires multiple iterations to arrive at the optimal conditions. Our experts will work closely with you to resolve any issues in your research project.

  • strategy optimization (Scaling Up)

    If you need to large scale custom protein production, we will create a custom strategy based on the optimal conditions we identified in the pilot expression.

  • Scale up production (Scaling Up)

    Our bioreactors stand ready to scale up production for you. We can be your large scale protein production partner and produce your target protein in grams levels or even more.

 

Why Choose Boster For Recombinant Protein Expression?

High-efficiency custom protein production service for all your needs.

Established in 1993, Boster Bio offers one of the most efficient recombinant protein services—we are one of the biggest antibody companies who use recombinant immunogen to create antibodies. We express 3000+ recombinant proteins each year for our antibody immunization. This has polished every aspect of our recombinant protein expression and purification process from negotiating the lowest possible protein synthesis cost to resolving tricky solubility issues.

AFFINITY TAGS AND FUSION PROTEINS

Insoluble or otherwise difficult to purify recombinant proteins are often labelled with "tags", or joined with other proteins as "fusion proteins". These fused products facilitate the purification and recovery thereof via affinity purification with a stationary phase that has an affinity for the tag or fused protein. Tags may be added to either the N- or C-terminus of the expression protein. Tagged systems may be used to avoid many of the difficulties encountered during protein purification.

Examples of highly popular pairs are the His-tag (poly histidine tag), the MBP-tag (maltose binding protein), and the GST-tag (glutathione-S-transferase). These tags are typically utilized to render a soluble protein product that is normally expressed in an insoluble form, and other desirable purification enhancers. Affinity purification such as nickel-chelate chromatography is commonly used with His-tag proteins, as they have high affinity and specificity for the ions of nickel and other metals. Finally, the tag must be easily removed after purification to ensure no effect on the protein's function or activity upon analysis.

FAQS About Protein Expression

 

 

Yes, our protein expression services include full-service protein expression with all common downstream services, such as protein synthesis, protein purification, and desalting, and also serve manufacturing needs, such as lyophilization, packaging and labeling.

I am assuming you are asking how to work with us. In that case, to get started you can tell us the protein sequence you want to express, along with any special requests you might have. We will make a free quote for you with which you can place a purchase order. Please enter a valid email address, and once we receive a purchase order under your company name, we will optimize the codons for you and take it from there. The detailed milestones and other product information can be found in the above section

Yes we can. It will have to be one of the advanced expression package with eukaryotic expression systems (yeast, baculovirus insect cell expression system or mammalian expression systems). Also not all PTMs can be achieved, we need to know more details about your PTM of interest.

Yes, as long as the expression vector is not under patent/IP protection in the United States, United Kingdom, or any other country, or if you can extend your license to us. If there are no legal limitations, technically we can accommodate your expression vector.

A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses.

The most popular expression systems for recombinant protein expression are bacterial, yeast, insect/baculovirus, and mammalian systems.

Protein expression is often quantified by common immunoassays, such as ELISA (enzyme-linked immunosorbent assay) or western blotting.