Western Blot Reagents

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  1. 1M Tris-HCI, pH6.8 AR1163
    $10.00
    • Western Blotting Reagents
    • 100ml
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    Boster’s 1M Tris-HCI,pH6.8 is the commonly used reagent for SDS-PAGE electrophoresis to prepare acrylamide gels. Learn More
  2. 1.5M Tris-HCI, pH8.8 AR1162
    $10.00
    • Western Blotting Reagents
    • 100ml
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    Boster’s 1.5M Tris-HCI,pH8.8 is the commonly used reagent for SDS-PAGE electrophoresis to prepare acrylamide gels. Learn More
  3. Mammalian Tissue Protein Extraction Reagent AR0101
    $100.00
    • Western Blotting Reagents, Sample Preparation
    • 100 mL (100 assays for 0.1g of tissuе)
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    Boster’s Mammalian Tissue Protein Extraction Reagent is a ready-to-use Western blot related reagent solution used for efficient extraction of total soluble protein in nondenatured state from animal tissues. Learn More
  4. ISH Detection Kit (POD) MK1030
    $150.00
    • Western Blotting Reagents, Sample Preparation
    • 1 kit (sufficient for staining100 slides)
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    ISH Detection Kit Learn More
  5. Bovine Serum Albumin Standard AR0191
    $20.00
    • Western Blotting Reagents, Sample Preparation
    • 20mL
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    Bovine Serum Albumin Standard is a ready-to-use solution for determining protein concentration Learn More
  6. ECL Plus Western Blotting Substrate AR1196
    $50.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 50mL
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    Boster's ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). Boster Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film. Learn More
  7. BCA Protein Assay Kit AR0146-1L
    $400.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
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    BCA Protein Assay Kit, Western Blotting Related Reagent Learn More
  8. BCA Protein Assay Kit AR0146-500
    $230.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
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    BCA Protein Assay Kit, Western Blotting Related Reagent Learn More
  9. 30% Acrylamide/Bis Solution, 29:1 AR1161
    $50.00
    • Western Blotting Reagents
    • 500ml
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    30% Acrylamide/Bis Solution, 29:1, Assistant Reagent Learn More
  10. Broad Spectrum Protease Inhibitor Cocktail (EDTA free) (100x) AR1182-1
    $50.00
    • Western Blotting Reagents, Sample Preparation
    • 1ml
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    Boster’s Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.  Learn More

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Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

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