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Gold Standard Quantitative or Real-TIme PCR Services
Quantitative PCR Services
Quantitative Real-time PCR (qPCR) is the gold standard for the precise nucleic acids quantification in genomics research, biologics testing and drug discovery. Our qPCR experts are excited to accelerate your research with Boster’s Fast, sensitive, and reliable service.
Get a Quote
Free Quote For qPCR Services
Fill the form below to get a quote. Contact us at [email protected] for any questions.
SOW options explained
Return or despose excess materials: The remaining samples and reagents be shipped back to you or disposed of. There are fees associated with both options
Sample replication: by default we assume all samples are tested in triplicates.
Special requirements: anything not covered in the SOW template and the sample manifest can be written here.
Quantitative PCR Applications
Gene Expression
Identification of tumor cell lines for drug efficacy
DNA Quantification
Accurate copy number variation analysis
Biomarker Screening
Predicting a drug's response in animal or cellular models
Pharmacodynamic Determination
Analysis of time or dose dependency and mode of action
Genotyping
Stratification marker verification and allelic discrimination
Diagnostics
Pathogen detection and quantification
Boster QPCR Advantages
End-to-end Workflow
from sample prep to data analysis
Assay Development Experts
qPCR primer design, optimization, and troubleshooting
High Throughput Capacity
qPCR solutions to rapidly scale pilot or exploratory projects
Complete CRO Service
qPCR combined with Boster's other services for a complete CRO service
How to Order Boster’s qPCR Service
As easy as 1, 2, 3.
1. Free Project Consultation with our Expert Scientists
2. Send Samples
3. Receive a Comprehensive Analysis Report
qPCR Services TImeline
qPCR Sample Requirements
| Sample Format | Details |
|---|---|
| Total RNA | RNA isolated from >1 million cells supplied in 1 ml TRIzol solution, please ship on dry ice |
| Cell Pellet | Please prepare from >1 million mammalian cells, please ship on dry ice |
| cDNA | 2ug of cDNA for each gene to be tested per sample (in TE buffer, at least 100 ng/ul) |
| Primers/Probes | 100 ul of primers/probes at 100uM per target gene |
We promise You a concierge sourcing experience
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It is our project concierges' mission to make your project experience as smooth and memorable as possible. They are subject matter experts who are easily accessible around the clock, always happy to help you solve problems, make recommendations and sort through options.
Sample Report
What is Quantitative or Real Time PCR?
Real-Time PCR (RT-PCR) or Quantitative PCR (qPCR) is a sensitive and reproducible method of determining the levels of gene expression in an organism. Simply put it is a way of finding out how much of a specific section of DNA is in a sample, in Real Time. Widely known as the “Gold Standard” for DNA and RNA quantification and genotyping, it can be used in the drug discovery process to quantify changes in mRNA expression of drug targets, PD markers or predictive biomarkers.
qPCR builds on the principal of PCR where amplification is achieved using nucleotides and primers, with fluorescent dyes or DNA probes added to detect PCR products as they are produced. Specific nucleic acids can be quantified by Absolute Quantification, by comparing the sample to a DNA standard to measure the exact number of target DNA molecules, or Relative Quantification using internal reference genes within the sample to determine the quantity of the target gene.
qPCR provides fast and high-throughput detection and quantification of target DNA sequences by lowering amplification and allowing simultaneous visualization of all newly formed DNA amplicons.
qPCR Related Services
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ELISA Services
Boster Bio will test your samples and provide you scientific consultation, backed by our network of experts with experience in your analytes.
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Western Blot Services
Boster offers competitively priced Western blot services with quick turnaround. We also provide 2D-WB and LC-MS for advanced characterization.
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IHC/IF Services
Boster offers competitively priced histology/IHC/IF services with quick turnaround. Featuring a large selection of FFPE blocks and tissue/cell arrays.
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Compound Screening Services
Ready-to-go screening assays to ensure your discovery process stays on track from the earliest discovery stages.
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Rabbit Monoclonal Antibody Service
Get fully validated, affinity matured rabbit monoclonal antibodies using our patented microfluidics PCD platform.
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NGS Services
Boster Bio offers a full range of quality NGS services extending from nucleic acid extraction, to library preparation, sequencing and bioinformatic analysis.
FAQs
Q1. What are the applications of qPCR?
There are multiple research applications based on qPCR. These include
gene expression analysis, genotyping, allelic discrimination, single nucleotide polymorphism (SNP)
detection, microbe and pathogen detection, species abundance quantification and many others. Boster’s
applications can be found here
Q2. What is the difference between PCR and qPCR?
PCR is the traditional amplification method used to identify the
presence or absence of a DNA fragment. QPCR is used to quantify a particular fragment in a sample. Thus, PCR
is a qualitative technique whereas qPCR is a quantitative technique. More information about qPCR can be
found here
Q3. What are the sample requirements for my qPCR assay?
Sample requirements can vary depending on your project requirements
and we would always recommend speaking with our Project Concierge, but as a general
guideline please check our Sample Requirements as per this link
Q4. What are the key benefits of qPCR assays?
qPCR is a way of finding out how much of a specific section of DNA is
in a sample, in Real Time. qPCR provides fast and high-throughput detection and quantification of target DNA
sequences. Amplification time is lower and allowing simulaneous visualization of all newly formed DNA
amplicons. More information on the key benefits can be found here