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Product Info Summary
| SKU: | A00131-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Tlr2 Antibody Picoband®
SKU/Catalog Number
A00131-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tlr2 Antibody Picoband® catalog # A00131-4. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tlr2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00131-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Tlr2 recombinant protein (Position: K20-K683).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00131-4 is reactive to Tlr2 in Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
89838 MW
Background of TLR2
Toll-like receptor 2 also known as TLR2 is a protein that in humans is encoded by the TLR2 gene. Enables lipoteichoic acid binding activity and peptide binding activity. Involved in several processes, including negative regulation of cellular component organization; positive regulation of cytokine production; and positive regulation of macromolecule biosynthetic process. Acts upstream of or within several processes, including pattern recognition receptor signaling pathway; regulation of cytokine production; and response to bacterium. Located in external side of plasma membrane. Part of Toll-like receptor 2-Toll-like receptor 6 protein complex. Is expressed in several structures, including brain; early embryo; genitourinary system; gut; and immune system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); dermatitis (multiple); gastrointestinal system cancer (multiple); lung disease (multiple); and rhinitis (multiple). Orthologous to human TLR2 (toll like receptor 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00131-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tlr2 using anti-Tlr2 antibody (A00131-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr2 antigen affinity purified polyclonal antibody (Catalog # A00131-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr2 at approximately 89 kDa. The expected band size for Tlr2 is at 89 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Tlr2 (Source: Uniprot.org, NCBI)
Gene Name
Tlr2
Full Name
Toll-like receptor 2
Weight
89838 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; TLR2 CD282, TIL4 toll like receptor 2 toll-like receptor 2|toll/interleukin-1 receptor-like protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tlr2, check out the Tlr2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tlr2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tlr2 Antibody Picoband® (A00131-4)
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