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Product Info Summary
| SKU: | EK1130 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1130
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1130)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q25-L590
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1130 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1130 is reactive to Tlr2 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1130 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TLR2
Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1130-CAP | Anti-Mouse Tlr2 Pre-coated 96-well strip microplate | 1 |
| EK1130-ST | Mouse Tlr2 Standard | 2 vials, 10 ng/tube |
| EK1130-DA | Mouse Tlr2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.016 | 0.078 | 0.128 | 0.200 | 0.412 | 0.853 | 1.434 | 2.097 |
Data Example Images
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Mouse TLR2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 304 | 1448 | 4661 | 301 | 1423 | 4925 |
| Standard deviation | 21.58 | 65.76 | 195.76 | 26.48 | 91.07 | 280.72 |
| CV (%) | 7.1% | 4.5% | 4.2% | 8.8% | 6.4% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TLR2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 304 | 323 | 310 | 307 | 311 | 7.24 | 2.3% |
| Sample 2 | 1448 | 1379 | 1487 | 1331 | 1411 | 60.35 | 4.2% |
| Sample 3 | 4661 | 5150 | 4522 | 4797 | 4782 | 233.39 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For Tlr2 (Source: Uniprot.Org, NCBI)
Gene Name
Tlr2
Full Name
Toll-like receptor 2
Weight
89449 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 2;CD282;Tlr2; TLR2 CD282, TIL4 toll like receptor 2 toll-like receptor 2|toll/interleukin-1 receptor-like protein 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tlr2, check out the Tlr2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tlr2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (EK1130)
Hello CJ!
EK1130 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
HuR induces inflammatory responses in HUVECs and murine sepsis via binding to HMGB1
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TLR2 using the EK1130 Mouse TLR2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-03
Question
What antibody the mouse is producing would be detected by your Mouse TLR2 ELISA Kit PicoKine after injecting the vaccine into a mouse that was made in CHO cells?
Verified customer
Asked: 2019-11-26
Answer
Unfortunately, Mouse TLR2 ELISA Kit PicoKine is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.
Boster Scientific Support
Answered: 2019-11-26
Question
Q: is it okay to use citrate plasma as samples in Mouse TLR2 Picokine® ELISA Kit (Catalog # EK1130)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TLR2 causing disruption of its protein structure. TLR2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TLR2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-13
Question
Q: how to proceed with the analysis of ELISA data? I have obtained TLR2 level in serum.
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-10-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Z. Anderson
Verified customer
Asked: 2017-06-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-06-18
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
H. Singh
Verified customer
Asked: 2014-07-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-07-07
Question
We have received a question regarding EK1130 and I am emailing to see if you can help. Here is the customer
Verified Customer
Verified customer
Asked: 2013-03-14
Answer
EK1130 is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.
Boster Scientific Support
Answered: 2013-03-14
