Click image to see more details
Product Info Summary
| SKU: | M00131 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TLR2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00131
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TLR2 Rabbit Monoclonal Antibody catalog # M00131. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00131)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TLR2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00131 is reactive to TLR2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
89838 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00131 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (M00131).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified monoclonal antibody (Catalog # M00131) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 90 kDa. The expected band size for TLR2 is at 90 kDa.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For TLR2 (Source: Uniprot.org, NCBI)
Gene Name
TLR2
Full Name
Toll-like receptor 2
Weight
89838 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; TLR2 CD282, TIL4 toll like receptor 2 toll-like receptor 2|toll/interleukin-1 receptor-like protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR2, check out the TLR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR2 Rabbit Monoclonal Antibody (M00131)
Hello CJ!
M00131 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi Y,Vistro WA,Bai X,Wu R,Chen C,Huang Y,Fazlani SA,Tarique I,Yang P,Chen Q.Effect of seasonal variance on intestinal epithelial barriers and the associated innate immune response of the small intestine of the Chinese soft-shelled turtles.Fish Shellfish
Species: Turtles
M00131 usage in article: APP:WB, SAMPLE:SMALL INTESTINE, DILUTION:1:75
Feng S,Ju L,Shao Z,Grzanna M,Jia L,Liu M.Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis.J Inflamm Res.2020 Jul 21;13:343-356.doi:10.2147/JIR.S254717.PMID: 32801828;PMCID:PMC7398876.
Species: Mouse
M00131 usage in article: APP:WB, SAMPLE:SPLEEN, DILUTION:NA
Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft%u2010Shelled Turtle Pelodiscus sinensis During the Hibernation Season
Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers
Wang Y, Ge P, Yang L, Wu C, Zha H, Luo T, Zhu Y. J Neuroinflammation. 2014 Jan 24;11:15. Doi: 10.1186/1742-2094-11-15. Protection Of Ischemic Post Conditioning Against Transient Focal Ischemia-Induced Brain Damage Is Associated With Inhibition Of ...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TLR2 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TLR2 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-TLR2 Rabbit Monoclonal Antibody
Question
We are currently using anti-TLR2 Rabbit Monoclonal antibody M00131 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The anti-TLR2 Rabbit Monoclonal antibody (M00131) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
I am interested in to test anti-TLR2 Rabbit Monoclonal antibody M00131 on mouse monocyte for research purposes, then I may be interested in using anti-TLR2 Rabbit Monoclonal antibody M00131 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The products we sell, including anti-TLR2 Rabbit Monoclonal antibody M00131, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-TLR2 Rabbit Monoclonal antibody M00131 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-10
Question
See below the WB image, lot number and protocol we used for monocyte using anti-TLR2 Rabbit Monoclonal antibody M00131. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
My team were content with the WB result of your anti-TLR2 Rabbit Monoclonal antibody. However we have seen positive staining in fetal lung membrane using this antibody. Is that expected? Could you tell me where is TLR2 supposed to be expressed?
K. Zhang
Verified customer
Asked: 2019-11-19
Answer
From literature, fetal lung does express TLR2. Generally TLR2 expresses in membrane. Regarding which tissues have TLR2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Fetal lung, Pubmed ID: 9751057
Leukocyte, and Prostate, Pubmed ID: 9596645, 9435236
Monocyte, Pubmed ID: 16622205
T-cell, Pubmed ID: 10426995, 21078852
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-TLR2 Rabbit Monoclonal antibody M00131 work for IHC with monocyte?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
According to the expression profile of monocyte, TLR2 is highly expressed in monocyte. So, it is likely that anti-TLR2 Rabbit Monoclonal antibody M00131 will work for IHC with monocyte.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-TLR2 Rabbit Monoclonal antibody (M00131)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do provide the blocking peptide for product anti-TLR2 Rabbit Monoclonal antibody (M00131). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-TLR2 Rabbit Monoclonal antibody M00131. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
Can you help my question with product M00131, anti-TLR2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00131 anti-TLR2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-12
Question
We have been able to see staining in rat blood. Do you have any suggestions? Is anti-TLR2 Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Based on literature blood does express TLR2. Based on Uniprot.org, TLR2 is expressed in blood, leukocyte prostate, fetal lung, t-cell, monocyte, among other tissues. Regarding which tissues have TLR2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Fetal lung, Pubmed ID: 9751057
Leukocyte, and Prostate, Pubmed ID: 9596645, 9435236
Monocyte, Pubmed ID: 16622205
T-cell, Pubmed ID: 10426995, 21078852
Boster Scientific Support
Answered: 2019-07-19
Question
Will anti-TLR2 Rabbit Monoclonal antibody M00131 work on zebrafish IHC with monocyte?
P. Bhatt
Verified customer
Asked: 2019-05-10
Answer
Our lab technicians have not validated anti-TLR2 Rabbit Monoclonal antibody M00131 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TLR2 Rabbit Monoclonal antibody M00131 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish monocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-10
Question
I am looking for using your anti-TLR2 Rabbit Monoclonal antibody for response to progesterone studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-30
Answer
Thanks for your inquiry. This M00131 anti-TLR2 Rabbit Monoclonal antibody is validated on a549 cell lysate, hela cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-30
Question
I was wanting to use your anti-TLR2 Rabbit Monoclonal antibody for IHC for mouse monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse monocyte identification?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
It shows on the product datasheet, M00131 anti-TLR2 Rabbit Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-19
Question
Is this M00131 anti-TLR2 Rabbit Monoclonal antibody reactive to the isotypes of TLR2?
G. Anderson
Verified customer
Asked: 2016-12-22
Answer
The immunogen of M00131 anti-TLR2 Rabbit Monoclonal antibody is A synthesized peptide derived from human TLR2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-22
Question
Will M00131 anti-TLR2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Evans
Verified customer
Asked: 2014-08-25
Answer
It shows on the product datasheet, M00131 anti-TLR2 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-25
Question
We bought anti-TLR2 Rabbit Monoclonal antibody for IHC on leukocyte prostate a few months ago. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining leukocyte prostate as well as fetal lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
S. Parker
Verified customer
Asked: 2014-04-14
Answer
I have checked the website and datasheets of our anti-TLR2 Rabbit Monoclonal antibody and it seems that M00131 has been validated on human in both IHC and WB. Thus M00131 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-04-14
Question
Is there a BSA free version of anti-TLR2 Rabbit Monoclonal antibody M00131 available?
D. Jones
Verified customer
Asked: 2013-06-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TLR2 Rabbit Monoclonal antibody M00131 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-06-13
