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Product Info Summary
| SKU: | A00131 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TLR2 Antibody Picoband®
SKU/Catalog Number
A00131
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TLR2 Antibody Picoband® catalog # A00131. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00131)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TLR2 recombinant protein (Position: H610-S784). Human TLR2 shares 87.4% amino acid (aa) sequence identity with mouse TLR2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00131 is reactive to TLR2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
105 kDa
Calculated molecular weight
89838 MW
Background of TLR2
Toll-like receptor 2, also known as TLR-2, is a protein that in humans is encoded by the TLR2 gene. It has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. And TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00131 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human THP-1 (-LPS) whole cell, human THP-1 (+LPS) whole cell, human Hacat whole cell, human PC-3 whole cell, human Caco-2 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (A00131).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 (-LPS) whole cell lysates,
Lane 2: human THP-1 (+LPS) whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (A00131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 105 kDa. The expected band size for TLR2 is at 90 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TLR2 (Source: Uniprot.org, NCBI)
Gene Name
TLR2
Full Name
Toll-like receptor 2
Weight
89838 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; TLR2 CD282, TIL4 toll like receptor 2 toll-like receptor 2|toll/interleukin-1 receptor-like protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR2, check out the TLR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR2 Antibody Picoband® (A00131)
Hello CJ!
A00131 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Upregulation of Toll‑like receptor 2 expression in colorectal cancer infected by human cytomegalovirus
Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus
Protective Effects of Selenium Nanoparticle-Enriched Lactococcus lactis NZ9000 against Enterotoxigenic Escherichia coli K88-Induced Intestinal Barrier Damage in Mice | Applied and Environmental Microbiology
Shikonin inhibits the cell viability, adhesion, invasion and migration of the human gastric cancer cell line MGC‐803 via the Toll‐like receptor 2/nuclear factor‐kappa B pathway
Effect of lentivirus-mediated gene silencing, targeting toll-like receptor 2, on corneal allograft transplantation in rats
Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways
Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft‐Shelled Turtle Pelodiscus sinensis During the Hibernation Season
Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft‐Shelled Turtle Pelodiscus sinensis During the Hibernation Season
Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft‐Shelled Turtle Pelodiscus sinensis During the Hibernation Season
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TLR2 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte prostate using anti-TLR2 antibody A00131. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
We have seen staining in rat leukocyte prostate. What should we do? Is anti-TLR2 antibody supposed to stain leukocyte prostate positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature leukocyte prostate does express TLR2. From Uniprot.org, TLR2 is expressed in blood, leukocyte prostate, fetal lung, t-cell, monocyte, among other tissues. Regarding which tissues have TLR2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Fetal lung, Pubmed ID: 9751057
Leukocyte, and Prostate, Pubmed ID: 9596645, 9435236
Monocyte, Pubmed ID: 16622205
T-cell, Pubmed ID: 10426995, 21078852
Boster Scientific Support
Answered: 2020-02-10
Question
Does A00131 anti-TLR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
As indicated on the product datasheet, A00131 anti-TLR2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-16
Question
My lab would like to test anti-TLR2 antibody A00131 on rat leukocyte prostate for research purposes, then I may be interested in using anti-TLR2 antibody A00131 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The products we sell, including anti-TLR2 antibody A00131, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-09
Question
We are currently using anti-TLR2 antibody A00131 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-TLR2 antibody (A00131) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this A00131 anti-TLR2 antibody reactive to the isotypes of TLR2?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The immunogen of A00131 anti-TLR2 antibody is E.coli-derived human TLR2 recombinant protein (Position: H610-S784). Human TLR2 shares 87.4% amino acid (aa) sequence identity with mouse TLR2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-13
Question
Does anti-TLR2 antibody A00131 work for WB with leukocyte prostate?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
According to the expression profile of leukocyte prostate, TLR2 is highly expressed in leukocyte prostate. So, it is likely that anti-TLR2 antibody A00131 will work for WB with leukocyte prostate.
Boster Scientific Support
Answered: 2019-05-20
Question
I have a question about product A00131, anti-TLR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00131 anti-TLR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-23
Question
We are interested in using your anti-TLR2 antibody for cellular response to bacterial lipopeptide studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
I appreciate your inquiry. This A00131 anti-TLR2 antibody is validated on rat spleen tissue, mouse spleen tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-24
Question
We were well pleased with the WB result of your anti-TLR2 antibody. However we have been able to see positive staining in t-cell membrane using this antibody. Is that expected? Could you tell me where is TLR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
From literature, t-cell does express TLR2. Generally TLR2 expresses in membrane. Regarding which tissues have TLR2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Fetal lung, Pubmed ID: 9751057
Leukocyte, and Prostate, Pubmed ID: 9596645, 9435236
Monocyte, Pubmed ID: 16622205
T-cell, Pubmed ID: 10426995, 21078852
Boster Scientific Support
Answered: 2018-03-12
Question
I was wanting to use your anti-TLR2 antibody for WB for rat leukocyte prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukocyte prostate identification?
H. Rodriguez
Verified customer
Asked: 2018-02-15
Answer
As indicated on the product datasheet, A00131 anti-TLR2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-15
Question
Is a blocking peptide available for product anti-TLR2 antibody (A00131)?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
We do provide the blocking peptide for product anti-TLR2 antibody (A00131). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-18
Question
Do you have a BSA free version of anti-TLR2 antibody A00131 available?
B. Rodriguez
Verified customer
Asked: 2017-07-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TLR2 antibody A00131 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-25
Question
See attached the WB image, lot number and protocol we used for leukocyte prostate using anti-TLR2 antibody A00131. Please let me know if you require anything else.
K. Yang
Verified customer
Asked: 2014-08-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-11
Question
I see that the anti-TLR2 antibody A00131 works with WB, what is the protocol used to produce the result images on the product page?
W. Li
Verified customer
Asked: 2013-05-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-22
