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Product Info Summary
| SKU: | EK0843 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TNFSF11/RANKL ELISA Kit PicoKine®
View all TRANCE/TNFSF11/RANK L ELISA kits
SKU/Catalog Number
EK0843
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFSF11/RANKL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFSF11/RANKL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0843)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: R72-D316
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0843 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0843 is reactive to Tnfsf11 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0843 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRANCE/TNFSF11/RANK L
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0843-CAP | Anti-Mouse Tnfsf11 Pre-coated 96-well strip microplate | 1 |
| EK0843-ST | Mouse Tnfsf11 Standard | 2 vials, 10 ng/tube |
| EK0843-DA | Mouse Tnfsf11 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.040 | 0.118 | 0.154 | 0.285 | 0.430 | 0.792 | 1.322 | 2.032 |
Data Example Images
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Mouse TNFSF11/RANKL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum and plasma is 30-290 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFSF11/RANKL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 132 | 430 | 32 | 199 | 419 |
| Standard deviation | 1.42 | 10.72 | 17.63 | 1.72 | 12.13 | 21.36 |
| CV (%) | 4.2% | 5.5% | 4.1% | 5.4% | 6.1% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 39 | 32 | 37 | 35 | 2.69 | 7.6% |
| Sample 2 | 195 | 191 | 201 | 186 | 193 | 5.49 | 2.8% |
| Sample 3 | 430 | 475 | 480 | 482 | 466 | 21.37 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For Tnfsf11 (Source: Uniprot.Org, NCBI)
Gene Name
Tnfsf11
Full Name
Tumor necrosis factor ligand superfamily member 11
Weight
35003 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 11;Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254;Tumor necrosis factor ligand superfamily member 11, membrane form;Tumor necrosis factor ligand superfamily member 11, soluble form;Tnfsf11;Opgl, Rankl, Trance; TNFSF11 CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf TNF superfamily member 11 tumor necrosis factor ligand superfamily member 11|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand 6B|tumor necrosis factor superfamily member 11
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tnfsf11, check out the Tnfsf11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFSF11/RANKL ELISA Kit PicoKine® (EK0843)
Hello CJ!
EK0843 has been cited in 28 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LAPTM5 is transactivated by RUNX2 and involved in RANKL trafficking in osteoblastic cells
Effects of Taxifolin on Osteoclastogenesis in vitro and in vivo
Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss
Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet
REV-ERB agonism suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss partially via FABP4 upregulation
Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss
Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson’s disease model mice
NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis
Lipoxin A4 suppresses osteoclastogenesis in RAW264.7 cells and prevents ovariectomy-induced bone loss
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse TNFSF11/RANKL ELISA Kit PicoKine®
Question
Does EK0843 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse TNFSF11/RANKL ELISA Kit PicoKine® (EK0843) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-07-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-11
Question
Q: Can TRANCE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-02-18
Question
Will EK0843 react with human samples?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
EK0843 does not react with human samples.
Boster Scientific Support
Answered: 2019-08-09
Question
Q: how to analyze ELISA data? I have performed TRANCE level in cell lysates.
C. Bhatt
Verified customer
Asked: 2019-01-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-26
Question
To Technical Support: I am interested to use this Mouse TNFSF11/RANKL ELISA Kit but I would like to know the minimum amount to run the sample. I will use na
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
It is not possible that we can predict the minimum amount to be use in the samples as the amount of RANKL varies between experimental populations. Stated in the datasheets the sensitivity of this Mouse TNFSF11/RANKL ELISA Kit is <10pg/ml, so if you know how much RANK is present in your sample then it would be possible to calculate the minimum amount. However, we can recommend to run the technique of quick ELISA with your samples. Quick ELISA is when you run your top, middle, bottom and blank standards in the first four wells of the first column on the plate. Then you can use the next four columns in the plate to set up a serial dilution of your samples. Just run the ELISA in those wells only and cut all the incubation times in half. Through this it would give you an idea of the dilution you need to use in order to get your samples to fall within the standard curve.
Boster Scientific Support
Answered: 2017-09-19
Question
As we know that RANKL presents on cell membrane cleaved by ADAM17 and in cytosol as soluble form. Could you tell me whether Mouse TNFSF11/RANKL ELISA Kit reacts with soluble form and membrane-binding form?
Verified Customer
Verified customer
Asked: 2017-04-20
Answer
The Mouse TNFSF11/RANKL ELISA Kit detects the membrane bound form of RANKL, however it does not detect the soluble form. Do check these publication for your further references; Rubin et al. 2004. Prostate Carcinoma Cells that Have Resided in Bone have an Upregulated IGF-IAxis. The Prostate 58:41-49. In this study they used the same clone antibody to detect the membrane bound form of RANKL (mRANKL) and an antibody from another source to detect the soluble form of RANKL (sRANKL)
Boster Scientific Support
Answered: 2017-04-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-11-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-11-26
