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Product Info Summary
| SKU: | A00340-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NFAT2/NFATC1 Antibody Picoband®
View all NFATC1/NFAT2 Antibodies
SKU/Catalog Number
A00340-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFAT2/NFATC1 Antibody Picoband® catalog # A00340-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFAT2/NFATC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00340-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFAT2/NFATC1 recombinant protein (Position: F374-E710).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00340-2 is reactive to NFATC1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
101.243kDa
Background of NFATC1/NFAT2
NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00340-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U20S whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NFAT2/NFATC1 using anti-NFAT2/NFATC1 antibody (A00340-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2/NFATC1 antigen affinity purified polyclonal antibody (Catalog # A00340-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT2/NFATC1 at approximately 120KD. The expected band size for NFAT2/NFATC1 is at 101KD.
Protein Target Info & Infographic
Gene/Protein Information For NFATC1 (Source: Uniprot.org, NCBI)
Gene Name
NFATC1
Full Name
Nuclear factor of activated T-cells, cytoplasmic 1
Weight
101.243kDa
Alternative Names
Nuclear factor of activated T-cells, cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-Atc; NFATc; NFATC1; NFAT2; NFATC NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc nuclear factor of activated T cells 1 nuclear factor of activated T-cells, cytoplasmic 1|NFAT transcription complex cytosolic component|nuclear factor of activated T-cells c|nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFATC1, check out the NFATC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFATC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFAT2/NFATC1 Antibody Picoband® (A00340-2)
Hello CJ!
A00340-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Herbal medicine Gan‑fu‑kang downregulates Wnt/Ca2+ signaling to attenuate liver fibrogenesis in vitro and in vivo
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Customer Q&As
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4 Customer Q&As for Anti-NFAT2/NFATC1 Antibody Picoband®
Question
We are currently using anti-NFAT2/NFATC1 antibody A00340-2 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The anti-NFAT2/NFATC1 antibody (A00340-2) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-24
Question
My boss were satisfied with the WB result of your anti-NFAT2/NFATC1 antibody. However we have observed positive staining in peripheral blood lymphocyte t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is NFATC1 supposed to be expressed?
K. Rodriguez
Verified customer
Asked: 2018-05-30
Answer
From what I have seen in literature, peripheral blood lymphocyte t-cell does express NFATC1. Generally NFATC1 expresses in cytoplasm. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2018-05-30
Question
We have observed staining in rat b-cell lymphoma. Do you have any suggestions? Is anti-NFAT2/NFATC1 antibody supposed to stain b-cell lymphoma positively?
A. Parker
Verified customer
Asked: 2015-04-24
Answer
From literature b-cell lymphoma does express NFATC1. From Uniprot.org, NFATC1 is expressed in muscle organ, peripheral blood lymphocyte t-cell, b-cell, b-cell lymphoma, brain, erythroleukemia, among other tissues. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2015-04-24
Question
We have tried in the past anti-NFAT2/NFATC1 antibody for ELISA on muscle organ in a previous project. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining muscle organ as well as peripheral blood lymphocyte t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
J. Krishna
Verified customer
Asked: 2013-05-14
Answer
I looked at the website and datasheets of our anti-NFAT2/NFATC1 antibody and it seems that A00340-2 has been validated on mouse in both ELISA and WB. Thus A00340-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-05-14
