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Product Info Summary
| SKU: | M00144-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD11b ITGAM Rabbit Monoclonal Antibody
SKU/Catalog Number
M00144-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b ITGAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00144-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GC-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD11b
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00144-1 is reactive to ITGAM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
127179 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00144-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: Raw2647 cell
IHC: human spleen tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of CD11b expression in RAW264.7 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD11b Antibody.
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Immunohistochemical analysis of paraffin-embedded mouse lung, using CD11b Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127179 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b ITGAM Rabbit Monoclonal Antibody (M00144-1)
Hello CJ!
M00144-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice
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Customer Q&As
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16 Customer Q&As for Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Question
My question regarding product M00144-1, anti-CD11b Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Jackson
Verified customer
Asked: 2020-04-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00144-1 anti-CD11b Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-30
Question
My boss were content with the WB result of your anti-CD11b Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to literature, liver does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-CD11b Rabbit Monoclonal antibody for IHC for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
As indicated on the product datasheet, M00144-1 anti-CD11b Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
Is this M00144-1 anti-CD11b Rabbit Monoclonal antibody reactive to the isotypes of ITGAM?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of M00144-1 anti-CD11b Rabbit Monoclonal antibody is A synthesized peptide derived from human CD11b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
Does anti-CD11b Rabbit Monoclonal antibody M00144-1 work on zebrafish IF with bone marrow cell?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-CD11b Rabbit Monoclonal antibody M00144-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD11b Rabbit Monoclonal antibody M00144-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish bone marrow cell in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD11b Rabbit Monoclonal antibody M00144-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-04
Question
We are currently using anti-CD11b Rabbit Monoclonal antibody M00144-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-CD11b Rabbit Monoclonal antibody (M00144-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
Question
I see that the anti-CD11b Rabbit Monoclonal antibody M00144-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-01
Question
Would M00144-1 anti-CD11b Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
It shows on the product datasheet, M00144-1 anti-CD11b Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-21
Question
Here is the WB image, lot number and protocol we used for blood using anti-CD11b Rabbit Monoclonal antibody M00144-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-21
Question
Do you have a BSA free version of anti-CD11b Rabbit Monoclonal antibody M00144-1 available?
W. Zhang
Verified customer
Asked: 2018-10-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD11b Rabbit Monoclonal antibody M00144-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-24
Question
Will anti-CD11b Rabbit Monoclonal antibody M00144-1 work for IHC with blood?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
According to the expression profile of blood, ITGAM is highly expressed in blood. So, it is likely that anti-CD11b Rabbit Monoclonal antibody M00144-1 will work for IHC with blood.
Boster Scientific Support
Answered: 2017-06-28
Question
We have tried in the past anti-CD11b Rabbit Monoclonal antibody for IF on blood in a previous project. I am using mouse, and I plan to use the antibody for IHC next. My question regards examining blood as well as bone marrow cell in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
A. Krishna
Verified customer
Asked: 2017-05-03
Answer
I have checked the website and datasheets of our anti-CD11b Rabbit Monoclonal antibody and I see that M00144-1 has been validated on mouse in both IF and IHC. Thus M00144-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-03
Question
Is a blocking peptide available for product anti-CD11b Rabbit Monoclonal antibody (M00144-1)?
N. Krishna
Verified customer
Asked: 2015-08-17
Answer
We do provide the blocking peptide for product anti-CD11b Rabbit Monoclonal antibody (M00144-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-17
Question
We have seen staining in mouse bone marrow cell. Any tips? Is anti-CD11b Rabbit Monoclonal antibody supposed to stain bone marrow cell positively?
J. Jones
Verified customer
Asked: 2013-11-11
Answer
According to literature bone marrow cell does express ITGAM. According to Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2013-11-11
Question
I would like to test anti-CD11b Rabbit Monoclonal antibody M00144-1 on mouse blood for research purposes, then I may be interested in using anti-CD11b Rabbit Monoclonal antibody M00144-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Krishna
Verified customer
Asked: 2013-06-26
Answer
The products we sell, including anti-CD11b Rabbit Monoclonal antibody M00144-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-06-26
