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Product Info Summary
| SKU: | PA1943-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD11b/ITGAM Antibody Picoband®
SKU/Catalog Number
PA1943-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD11b/ITGAM Antibody catalog # PA1943-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b/ITGAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1943-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD11b, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1943-1 is reactive to ITGAM in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
127179 MW
Background of ITGAM
ITGAM (Integrin Alpha-M), also called CD11B or Mo1 ALPHA SUBUNIT (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule. A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A; also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX). By in situ hybridization, Corbi et al. (1988) demonstrated that the genes encoding the alpha subunits of LFA1 (ITGAL), Mac1, and p150, 95 (ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11. Callen et al. (1991) narrowed the assignment to 16p11.2. Inflammation plays an essential role in the initiation and progression of atherosclerosis. Simon et al. (2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1943-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: JURKAT Whole Cell, RAJI Whole Cell
Validation Images & Assay Conditions
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Anti-CD11b antibody, PA1943-1, All Western blotting
All lanes: Anti-ITGAM(PA1943-1) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 170KD
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127179 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b/ITGAM Antibody Picoband® (PA1943-1)
Hello CJ!
PA1943-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Ren B, Zhang Yx, Zhou Hx, Sun Fw, Zhang Zf, Wei Z, Zhang Cy, Si Dw. J Neurol Sci. 2015 Jan 15;348(1-2):142-52. Doi: 10.1016/J.Jns.2014.11.026. Epub 2014 Nov 28. Tanshinone Iia Prevents The Loss Of Nigrostriatal Dopaminergic Neurons By Inhibiting N...
Zhao G, Wang S, Wang Z, Sun A, Yang X, Qiu Z, Wu C, Zhang W, Li H, Zhang Y, Zhao J, Zou Y, Ge J. Int J Cardiol. 2013 Sep 30;168(2):853-62. Doi: 10.1016/J.Ijcard.2012.10.022. Epub 2012 Nov 13. Cxcr6 Deficiency Ameliorated Myocardial Ischemia/Reperf...
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.
Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD11b/ITGAM Antibody Picoband®
Question
My colleagues were content with the WB result of your anti-CD11b/ITGAM antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
Based on literature, blood does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-06-13
Question
We are currently using anti-CD11b/ITGAM antibody PA1943-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
The anti-CD11b/ITGAM antibody (PA1943-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-08
Question
Our lab want to know about using your anti-CD11b/ITGAM antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on raji whole cell lysate? We would like to see some validation images before ordering.
E. Roberts
Verified customer
Asked: 2018-01-29
Answer
We appreciate your inquiry. This PA1943-1 anti-CD11b/ITGAM antibody is validated on jurkat whole cell lysate, raji whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-29
Question
We have been able to see staining in human bone marrow cell. Do you have any suggestions? Is anti-CD11b/ITGAM antibody supposed to stain bone marrow cell positively?
S. Li
Verified customer
Asked: 2014-10-10
Answer
From literature bone marrow cell does express ITGAM. From Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2014-10-10
