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Product Info Summary
| SKU: | A00144-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody
SKU/Catalog Number
A00144-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody catalog # A00144-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00144-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD11b recombinant protein (Position: N129-E336).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00144-Dyl488 is reactive to ITGAM in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
127.179kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00144-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127.179kDa
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b; ITGAM; CD11B; CR3A ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody (A00144-Dyl488)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody
Question
We have observed staining in human blood. What should we do? Is anti-Human CD11b DyLight® 488 conjugated antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
From what I have seen in literature blood does express ITGAM. From what I have seen in Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-04-06
Question
Does A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
Do you have a BSA free version of anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 available?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-05
Question
My team were happy with the WB result of your anti-Human CD11b DyLight® 488 conjugated antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
From literature, blood does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-12-20
Question
My lab would like to test anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 on human blood for research purposes, then I may be interested in using anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The products we sell, including anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-21
Question
I see that the anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-10
Question
See attached the WB image, lot number and protocol we used for blood using anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
K. Brown
Verified customer
Asked: 2019-08-07
Answer
The anti-Human CD11b DyLight® 488 conjugated antibody (A00144-Dyl488) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 work for Flow Cytometry with blood?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
According to the expression profile of blood, ITGAM is highly expressed in blood. So, it is likely that anti-Human CD11b DyLight® 488 conjugated antibody A00144-Dyl488 will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2019-07-24
Question
Is a blocking peptide available for product anti-Human CD11b DyLight® 488 conjugated antibody (A00144-Dyl488)?
M. Krishna
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-Human CD11b DyLight® 488 conjugated antibody (A00144-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
Is this A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody reactive to the isotypes of ITGAM?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The immunogen of A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody is E. coli-derived human CD11b recombinant protein (Position: N129-E336). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-28
Question
My question regarding product A00144-Dyl488, anti-Human CD11b DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Johnson
Verified customer
Asked: 2016-03-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-11
Question
I was wanting to use your anti-Human CD11b DyLight® 488 conjugated antibody for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
V. Taylor
Verified customer
Asked: 2014-02-04
Answer
You can see on the product datasheet, A00144-Dyl488 anti-Human CD11b DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-02-04
