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Product Info Summary
| SKU: | EK0695 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CD14 ELISA Kit PicoKine®
SKU/Catalog Number
EK0695
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD14 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD14 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0695)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A18-P345
Sensitivity
<10 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK0695 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0695 is reactive to Cd14 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0695 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD14
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0695-CAP | Anti-Mouse Cd14 Pre-coated 96-well strip microplate | 1 |
| EK0695-ST | Mouse Cd14 Standard | 2 vials, 50 ng/tube |
| EK0695-DA | Mouse Cd14 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.041 | 0.089 | 0.135 | 0.180 | 0.274 | 0.475 | 0.958 | 1.883 |
Data Example Images
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Mouse CD14 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD14, Dilution ratio of 1:1, concentration in serum and plasma is 3-8 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD14 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1809 | 4760 | 24340 | 1781 | 4305 | 24426 |
| Standard deviation | 103.11 | 261.8 | 1217 | 119.32 | 236.77 | 1392.28 |
| CV (%) | 5.7% | 5.5% | 5% | 6.7% | 5.5% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1809 | 1708 | 1610 | 1761 | 1722 | 73.87 | 4.2% |
| Sample 2 | 4760 | 4159 | 4699 | 4655 | 4568 | 239.2 | 5.2% |
| Sample 3 | 24340 | 25258 | 25930 | 26104 | 25408 | 692.82 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For Cd14 (Source: Uniprot.Org, NCBI)
Gene Name
Cd14
Full Name
Monocyte differentiation antigen CD14
Weight
39204 MW
Alternative Names
Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD14;Cd14; CD14 CD14 molecule monocyte differentiation CD14|myeloid cell-specific leucine-rich glycoprotein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd14, check out the Cd14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD14 ELISA Kit PicoKine® (EK0695)
Hello CJ!
EK0695 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Effect of Hops (Humulus lupulus L.) Extract Supplementation on Weight Gain, Adiposity and Intestinal Function in Ovariectomized Mice
Jiang Jx, Chen Yh, Xie Gq, Ji Sh, Liu Dw, Qiu J, Zhu Pf, Wang Zg. World J Surg. 2003 Jan;27(1):1-9. Intrapulmonary Expression Of Scavenger Receptor And Cd14 And Their Relation To Local Inflammatory Responses To Endotoxemia In Mice.
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Customer Reviews
Have you used Mouse CD14 ELISA Kit PicoKine®?
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1 Reviews For Mouse CD14 ELISA Kit PicoKine®
0
Excellent CD14 ELISA Kit From BosterBio for Barrier Dysfunction
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Starting Material | Serum |
| Tips | Follow the incubation times closely |
| Results Summary | Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS. |
"CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction."
Sweta Ghosh
Verified customer
Submitted 2024-08-29
Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
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13 Customer Q&As for Mouse CD14 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-12-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-12-16
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
W. Lee
Verified customer
Asked: 2020-06-12
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD14 using the EK0695 Mouse CD14 Picokine® ELISA Kit?
B. Garcia
Verified customer
Asked: 2019-09-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-09-27
Question
Q: how to analyze ELISA data? I measured CD14 level in serum.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-14
Question
Q: if the enzyme conjugated CD14 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD14 antigen?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-04
Question
Q: how can I thaw whole blood sample for CD14 ELISA after freezing?
S. Lopez
Verified customer
Asked: 2018-12-07
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD14 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-07
Question
Q: What is the optimal O.D. value for CD14 ELISA kit? I used your CD14 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD14 even though the O.D. values are not very high?
K. Li
Verified customer
Asked: 2018-09-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD14 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD14 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-09-14
Question
Q: Can CD14 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Gonzalez
Verified customer
Asked: 2018-08-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-05
Question
What is the suggested dilution ratio for EK0695 for serum samples.
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The suggested dilution ratio range for serum samples is 1:1 - 1:5.
Boster Scientific Support
Answered: 2018-07-11
Question
Q: is it okay to use citrate plasma as samples in Mouse CD14 Picokine® ELISA Kit (Catalog # EK0695)?
S. Kaur
Verified customer
Asked: 2017-08-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD14 causing degradation of its protein structure. CD14 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD14 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-08-26
Question
What is the concentration of CD14 in mouse serum samples?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
The concentration of CD14 in mouse plasma/ serum samples ranges from 1-20ng/ml.
Boster Scientific Support
Answered: 2017-06-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Johnson
Verified customer
Asked: 2016-11-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-11-11
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD14 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-10-26
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD14 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-10-26
