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Product Info Summary
| SKU: | A00144 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD11b/ITGAM Antibody Picoband®
SKU/Catalog Number
A00144
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD11b/ITGAM Antibody Picoband® catalog # A00144. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b/ITGAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00144)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD11b recombinant protein (Position: E260-T360). Mouse CD11b shares 82.2% amino acid (aa) sequence identity with human CD11b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00144 is reactive to Itgam in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
128 kDa
Calculated molecular weight
127481 MW
Background of ITGAM
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00144 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse brain tissue, HEPA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: HEPA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 128KD. The expected band size for CD11b is at 127KD.
Protein Target Info & Infographic
Gene/Protein Information For Itgam (Source: Uniprot.org, NCBI)
Gene Name
Itgam
Full Name
Integrin alpha-M
Weight
127481 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;CD11b;Itgam; ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Itgam, check out the Itgam Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Itgam: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b/ITGAM Antibody Picoband® (A00144)
Hello CJ!
A00144 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.
Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD11b/ITGAM Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
I was wanting to use your anti-CD11b/ITGAM antibody for WB for mouse bone marrow cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse bone marrow cell identification?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
You can see on the product datasheet, A00144 anti-CD11b/ITGAM antibody has been tested for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse bone marrow cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-03
Question
Is there a BSA free version of anti-CD11b/ITGAM antibody A00144 available?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11b/ITGAM antibody A00144 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-23
Question
I have a question about product A00144, anti-CD11b/ITGAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00144 anti-CD11b/ITGAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-03
Question
We have observed staining in mouse blood. Any tips? Is anti-CD11b/ITGAM antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
According to literature blood does express ITGAM. According to Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-09-23
Question
Is this A00144 anti-CD11b/ITGAM antibody reactive to the isotypes of ITGAM?
P. Li
Verified customer
Asked: 2019-05-30
Answer
The immunogen of A00144 anti-CD11b/ITGAM antibody is E. coli-derived mouse CD11b recombinant protein (Position: E260-T360). Mouse CD11b shares 82.2% amino acid (aa) sequence identity with human CD11b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144. Let me know if you need anything else.
H. Edwards
Verified customer
Asked: 2018-11-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-28
Question
We want using your anti-CD11b/ITGAM antibody for positive regulation of microglial cell mediated cytotoxicity studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
We appreciate your inquiry. This A00144 anti-CD11b/ITGAM antibody is tested on mouse brain. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-30
Question
We are currently using anti-CD11b/ITGAM antibody A00144 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
The anti-CD11b/ITGAM antibody (A00144) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-16
Question
Will anti-CD11b/ITGAM antibody A00144 work for WB with bone marrow cell?
D. Jackson
Verified customer
Asked: 2017-11-24
Answer
According to the expression profile of bone marrow cell, ITGAM is highly expressed in bone marrow cell. So, it is likely that anti-CD11b/ITGAM antibody A00144 will work for WB with bone marrow cell.
Boster Scientific Support
Answered: 2017-11-24
Question
I was wanting to use to test anti-CD11b/ITGAM antibody A00144 on mouse bone marrow cell for research purposes, then I may be interested in using anti-CD11b/ITGAM antibody A00144 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
The products we sell, including anti-CD11b/ITGAM antibody A00144, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-27
Question
Would A00144 anti-CD11b/ITGAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Patel
Verified customer
Asked: 2015-11-10
Answer
It shows on the product datasheet, A00144 anti-CD11b/ITGAM antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-10
Question
I see that the anti-CD11b/ITGAM antibody A00144 works with WB, what is the protocol used to produce the result images on the product page?
K. Rodriguez
Verified customer
Asked: 2014-03-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-07
Question
We were happy with the WB result of your anti-CD11b/ITGAM antibody. However we have seen positive staining in bone marrow cell cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
N. Banerjee
Verified customer
Asked: 2013-04-01
Answer
From literature, bone marrow cell does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2013-04-01
Question
Is a blocking peptide available for product anti-CD11b/ITGAM antibody (A00144)?
C. Zhang
Verified customer
Asked: 2013-01-18
Answer
We do provide the blocking peptide for product anti-CD11b/ITGAM antibody (A00144). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-18
