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Product Info Summary
| SKU: | A00144-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CD11b/ITGAM Antibody Picoband®
SKU/Catalog Number
A00144-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD11b/ITGAM Antibody Picoband® catalog # A00144-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b/ITGAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00144-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD11b recombinant protein (Position: N129-E336).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00144-1 is reactive to ITGAM in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
127481 MW
Background of ITGAM
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00144-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CD11b antibody (A00144-1).
Overlay histogram showing THP-1 cells stained with A00144-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (A00144-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127481 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b; ITGAM; CD11B; CR3A ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b/ITGAM Antibody Picoband® (A00144-1)
Hello CJ!
A00144-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.
Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CD11b/ITGAM Antibody Picoband®
Question
My question regarding product A00144-1, anti-CD11b/ITGAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00144-1 anti-CD11b/ITGAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-03
Question
We have observed staining in rat blood. What should we do? Is anti-CD11b/ITGAM antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
Based on literature blood does express ITGAM. Based on Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-01-28
Question
My lab would like using your anti-CD11b/ITGAM antibody for negative regulation of dopamine metabolic process studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.
C. Banerjee
Verified customer
Asked: 2020-01-17
Answer
We appreciate your inquiry. This A00144-1 anti-CD11b/ITGAM antibody is validated on rat thymus tissue, spleen tissue, mouse thymus tissue. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-17
Question
Is there a BSA free version of anti-CD11b/ITGAM antibody A00144-1 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11b/ITGAM antibody A00144-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
I was wanting to use your anti-CD11b/ITGAM antibody for ELISA for rat bone marrow cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat bone marrow cell identification?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
It shows on the product datasheet, A00144-1 anti-CD11b/ITGAM antibody has been validated for ELISA, Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-24
Question
I see that the anti-CD11b/ITGAM antibody A00144-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-05
Question
Would anti-CD11b/ITGAM antibody A00144-1 work for ELISA with bone marrow cell?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of bone marrow cell, ITGAM is highly expressed in bone marrow cell. So, it is likely that anti-CD11b/ITGAM antibody A00144-1 will work for ELISA with bone marrow cell.
Boster Scientific Support
Answered: 2019-07-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-18
Question
My team were satisfied with the WB result of your anti-CD11b/ITGAM antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
From what I have seen in literature, liver does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-CD11b/ITGAM antibody A00144-1 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The anti-CD11b/ITGAM antibody (A00144-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-23
Question
Will A00144-1 anti-CD11b/ITGAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
You can see on the product datasheet, A00144-1 anti-CD11b/ITGAM antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
Is PB9140 and A00144-1 suitable for IF application?
Verified customer
Asked: 2019-03-26
Answer
IF test results of the Anti-CD11b/ITGAM Antibody Picoband™ (PB9140) and Anti-CD11b/ITGAM Antibody Picoband™ (A00144-1) were not good. We do not recommend using the antibodies for IF application.
Boster Scientific Support
Answered: 2019-03-26
Question
Is this A00144-1 anti-CD11b/ITGAM antibody reactive to the isotypes of ITGAM?
S. Anderson
Verified customer
Asked: 2018-11-05
Answer
The immunogen of A00144-1 anti-CD11b/ITGAM antibody is E. coli-derived human CD11b recombinant protein (Position: N129-E336). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-05
Question
My lab would like to test anti-CD11b/ITGAM antibody A00144-1 on rat bone marrow cell for research purposes, then I may be interested in using anti-CD11b/ITGAM antibody A00144-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Banerjee
Verified customer
Asked: 2018-01-30
Answer
The products we sell, including anti-CD11b/ITGAM antibody A00144-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-30
Question
We have tried in the past anti-CD11b/ITGAM antibody for IHC on bone marrow cell a few years ago. I am using rat, and We are going to use the antibody for WB next. Our lab want to know about examining bone marrow cell as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
S. Anderson
Verified customer
Asked: 2017-06-20
Answer
I looked at the website and datasheets of our anti-CD11b/ITGAM antibody and it appears that A00144-1 has been tested on rat in both IHC and WB. Thus A00144-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-20
Question
See attached the WB image, lot number and protocol we used for bone marrow cell using anti-CD11b/ITGAM antibody A00144-1. Please let me know if you require anything else.
J. Kulkarni
Verified customer
Asked: 2016-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-23
Question
Is a blocking peptide available for product anti-CD11b/ITGAM antibody (A00144-1)?
G. Baker
Verified customer
Asked: 2015-07-01
Answer
We do provide the blocking peptide for product anti-CD11b/ITGAM antibody (A00144-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-01
