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Key=name, value=Laemmli Buffer: SDS-PAGE Sample Loading Buffer 2X (Reducing)
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Key=description, value=Laemmli Buffer: SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
Key=short_description, value=Laemmli Buffer: SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
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Overview

Product Name	Laemmli Buffer: SDS-PAGE Protein Loading Buffer 2X (Reducing)
SKU/Catalog Number	AR0131-20
Form	Liquid
Size	20mL
Contents	4% SDS, 20% glycerol, 200mM DTT, 0.01% bromophenol blue and 0.1 M Tris HCl, pH 6.8
Description	SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
Equivalent	Millipore Sigma (Product No. S3401); Bio-Rad (Product No. 1610737)
Cite This Product	Laemmli Buffer: SDS-PAGE Protein Loading Buffer 2X (Reducing) (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0131-20)
Storage	Upon receipt store at -20°C. It is stable at -20°C for one year. Product is shipped on ice.

Assay Principle

Using bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a ready-to-use 2X solution. It contains 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromophenol blue and 0.1 M Tris HCl. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 12 mL protein samples. And the whole sample preparation process can be finished within 5 minutes.

Assay Protocol

1. Equilibrate SDS-PAGE Protein Loading Buffer 2X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C.

2. Mix one volume of SDS-PAGE Protein Loading Buffer 2X with one volume of protein sample (i.e. add 1mL protein sample into 1 mL Loading Buffer).

3. Boil sample for 3-5 min.
Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 2X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min.

4. Allow sample to cool to room temperature.

5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.

6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel.

General Notes

1. SDS-PAGE Protein Loading Buffer 2X contains DTT which has a slightly irritating odor.

2. SDS-PAGE Protein Loading Buffer 2X should be completely dissolved before use.

3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

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SKU	AR0131-20
Pack size	20mL
Form	Liquid

Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.. Shipped in room temperature.
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