Citrate Buffer Powder

Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. Cited in 10 publication(s).

Product Info Summary

SKU: AR0024
Size: 1 pack

Overview

Product Name Citrate Buffer Pack
SKU/Catalog Number AR0024
Pack Size 2000mL/pack
Form Dry-blend powder, sufficient to make 2000mL of Citrate Buffer
Volume of Final Solution 2000 mL
pH6
Buffer Contents 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Composition Sodium citrate.H2O
Storage Store at room temperature. It is stable at room temperature for one year.
Application IHC-P, HIER, PEFF tissues
*Our Boster Guarantee covers the use of this product in the above tested applications.
Equivalent Abcam (Product No. ab64214, ab64236)
Description Boster’s Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
Cite This Product Citrate Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0024)

Chemical Information

CAS NumberTrisodium citrate68-04-2
Citric acid77-92-9
Molecular WeightTrisodium citrate258.07
Citric acid192.123
Molecular FormulaTrisodium citrateC6H5Na3O7
Citric acidC6H8O7
Chemical SourcesTrisodium citrateChEMBL: ChEMBL1355
ChemSpider: 5989
PubChem: 6224
Citric acidChEMBL: ChEMBL1261
ChemSpider: 305
PubChem: 311

Usage and Handling

See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher.
Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.

Validation Images & Assay Conditions

Hello CJ!

AR0024 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

SNX-2112 Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Non-Small Cell Lung Cancer by Downregulating Epithelial-Mesenchymal Transition via the Wnt/β-Catenin Signaling Pathway

Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer

Expression and prognostic value of E2F3 transcription factor in non‑small cell lung cancer

PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma

Flotillin‑2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma

Overexpression of malignant brain tumor domain containing protein 1 predicts a poor prognosis of prostate cancer

High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer

IL‑8 is upregulated in cervical cancer tissues and is associated with the proliferation and migration of HeLa cervical cancer cells

Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer

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2 Customer Q&As for Citrate Buffer Powder

Question

What is the weight AR0024 supplied in grams?

Verified customer

Asked: 2020-11-30

Answer

For the Citrate Buffer Powder (AR0024), the weight of the powder supplied is 23g. Buffer Contents: 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O

Boster Scientific Support

Answered: 2020-11-30

Question

Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?

Verified customer

Asked: 2019-04-18

Answer

Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.

Boster Scientific Support

Answered: 2019-04-19

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