Anti-TMEM88 Antibody Picoband™

Product Name

Anti-TMEM88 Antibody Picoband™

View all Tmem88 Antibodies

SKU/Catalog Number

A12951-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMEM88 Antibody Picoband™ catalog # A12951-1. Tested in ICC/IF, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TMEM88 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12951-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM88, which shares 86.7% amino acid (aa) sequence identity with mouse TMEM88.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12951-1 is reactive to Tmem88 in Human

Applications

A12951-1 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

Background of Tmem88

Predicted to enable PDZ domain binding activity. Involved in negative regulation of canonical Wnt signaling pathway; protein localization to plasma membrane; and protein stabilization. Located in cytosol and plasma membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TMEM88 using anti-TMEM88 antibody (A12951-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM88 antigen affinity purified polyclonal antibody (Catalog # A12951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM88 at approximately 22 kDa. The expected band size for TMEM88 is at 17 kDa.

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Figure 2. IF analysis of TMEM88 using anti-TMEM88 antibody (A12951-1).
TMEM88 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMEM88 Antibody (A12951-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Tmem88 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20025; MGC71744; TMEM88; TMEM88A; transmembrane protein 88

For more info on Tmem88, check out the Tmem88 Infographic

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In this infographic, you will see the following information for Tmem88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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