Anti-Occludin/OCLN Antibody Picoband®

Product Name

Anti-Occludin/OCLN Antibody Picoband®

View all Occludin Antibodies

SKU/Catalog Number

RP1057

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Occludin/OCLN Antibody catalog # RP1057. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Occludin/OCLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1057)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1057 is reactive to OCLN in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

59 kDa

Calculated molecular weight

59144 MW

Background of Occludin

Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1057 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: SW620 Whole Cell, COLO320 Whole Cell, A549 Whole Cell, 293T Whole CellU87 Whole Cell
IHC: Human Mammary Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For OCLN (Source: Uniprot.org, NCBI)

Alternative Names

Occludin;OCLN; Ocln|AI503564, O, Ocl|occludin|occludin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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