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Product Info Summary
| SKU: | A02547-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NRAMP1/SLC11A1 Antibody Picoband®
View all NRAMP1/SLC11A1 Antibodies
SKU/Catalog Number
A02547-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NRAMP1/SLC11A1 Antibody Picoband® catalog # A02547-3. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NRAMP1/SLC11A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02547-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02547-3 is reactive to SLC11A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-120 kDa
Calculated molecular weight
59.872kDa
Background of NRAMP1/SLC11A1
Natural resistance-associated macrophage protein 1 is a protein that in humans is encoded by the SLC11A1 gene. It is mapped to 2q35. This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02547-3 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human K562 whole cell, human THP-1 whole cell, human placenta tissue, human A549 whole cell, human Caco-2 whole cell, human HepG2 whole cell, human U2OS whole cell, human Hela whole cell,
IHC: human intestinal cancer tissue, mouse spleen tissue, rat spleen tissue
ICC/IF: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates
Lane 2: human THP-1 whole cell lysates
Lane 3: human placenta tissue lysates
Lane 4: human A549 whole cell lysates
Lane 5: human Caco-2 whole cell lysates
Lane 6: human HepG2 whole cell lysates
Lane 7: human U2OS whole cell lysates
Lane 8: human Hela whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC11A1 antigen affinity purified polyclonal antibody (Catalog # A02547-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC11A1 at approximately 90-120KD. The expected band size for SLC11A1 is at 60KD.
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Figure 2. IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).
SLC11A1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).
SLC11A1 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).
SLC11A1 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IF analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).
SLC11A1 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SLC11A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC11A1
Full Name
Natural resistance-associated macrophage protein 1
Weight
59.872kDa
Superfamily
NRAMP family
Alternative Names
Natural resistance-associated macrophage protein 1; NRAMP 1; Solute carrier family 11 member 1; SLC11A1; LSH; NRAMP; NRAMP1 SLC11A1 LSH, NRAMP, NRAMP1 solute carrier family 11 member 1 natural resistance-associated macrophage protein 1|NRAMP 1|solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1|solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1|solute carrier family 11 (sodium/phosphate symporters), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC11A1, check out the SLC11A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC11A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NRAMP1/SLC11A1 Antibody Picoband® (A02547-3)
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Customer Q&As
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7 Customer Q&As for Anti-NRAMP1/SLC11A1 Antibody Picoband®
Question
We are currently using anti-NRAMP1/SLC11A1 antibody A02547-3 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
A. Edwards
Verified customer
Asked: 2020-04-14
Answer
The anti-NRAMP1/SLC11A1 antibody (A02547-3) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-14
Question
Does anti-NRAMP1/SLC11A1 antibody A02547-3 work for ELISA with blood?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to the expression profile of blood, SLC11A1 is highly expressed in blood. So, it is likely that anti-NRAMP1/SLC11A1 antibody A02547-3 will work for ELISA with blood.
Boster Scientific Support
Answered: 2020-02-20
Question
I was wanting to use your anti-NRAMP1/SLC11A1 antibody for ELISA for mouse blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
It shows on the product datasheet, A02547-3 anti-NRAMP1/SLC11A1 antibody has been tested for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-17
Question
I see that the anti-NRAMP1/SLC11A1 antibody A02547-3 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-11
Question
I have a question about product A02547-3, anti-NRAMP1/SLC11A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02547-3 anti-NRAMP1/SLC11A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-14
Question
Is this A02547-3 anti-NRAMP1/SLC11A1 antibody reactive to the isotypes of SLC11A1?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The immunogen of A02547-3 anti-NRAMP1/SLC11A1 antibody is E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
Question
Would A02547-3 anti-NRAMP1/SLC11A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Brown
Verified customer
Asked: 2015-03-12
Answer
It shows on the product datasheet, A02547-3 anti-NRAMP1/SLC11A1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-12
