Anti-Occludin OCLN Antibody

Product Name

Anti-Occludin OCLN Antibody

View all Occludin Antibodies

SKU/Catalog Number

A01246

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Occludin OCLN Antibody (Catalog # A01246). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

OCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Occludin OCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01246)

Host

Rabbit

Contents

OCLN Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of OCLN are known to exist.

Reactive Species

A01246 is reactive to OCLN in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

59144 MW

Background of Occludin

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01246 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For OCLN (Source: Uniprot.org, NCBI)

Alternative Names

BLCPMG, Occludin Ocln|AI503564, O, Ocl|occludin|occludin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis