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Product Info Summary
| SKU: | EK1317 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human VE-Cadherin CDH5 ELISA Kit PicoKine®
View all VE-Cadherin ELISA kits
SKU/Catalog Number
EK1317
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human VE-Cadherin CDH5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human VE-Cadherin CDH5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1317)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D48-Q599
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1317 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1317 is reactive to CDH5 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1317 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VE-Cadherin
Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1317-CAP | Anti-Human CDH5 Pre-coated 96-well strip microplate | 1 |
| EK1317-ST | Human CDH5 Standard | 2 vials, 20 ng/tube |
| EK1317-DA | Human CDH5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.086 | 0.163 | 0.220 | 0.286 | 0.505 | 0.814 | 1.279 | 1.970 |
Data Example Images
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Human VE-Cadherin/CD144 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VE-Cadherin, Dilution ratio of 1:50, concentration in serum and plasma is around 200 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VE-Cadherin CDH5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 670 | 1989 | 7534 | 703 | 2069 | 6886 |
| Standard deviation | 40.87 | 159.7%4 | 587.65 | 49.21 | 190.34 | 667.94 |
| CV (%) | 6.1% | 7.8% | 7.8% | 7% | 9.2% | 9.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VE-Cadherin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 670 | 673 | 650 | 693 | 671 | 15.23 | 2.2% |
| Sample 2 | 1989 | 2083 | 2227 | 2118 | 2104 | 85.13 | 4% |
| Sample 3 | 7534 | 8066 | 8375 | 7593 | 7892 | 346.81 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For CDH5 (Source: Uniprot.Org, NCBI)
Gene Name
CDH5
Full Name
Cadherin-5
Weight
87528 MW
Alternative Names
Cadherin-5;7B4 antigen;Vascular endothelial cadherin;VE-cadherin;CD144;CDH5; CDH5 7B4, CD144 cadherin 5 cadherin-5|7B4 |VE-cadherin|cadherin 5, type 2 (vascular endothelium)|cadherin 5, type 2, VE-cadherin (vascular epithelium)|cd144 |endothelial-specific cadherin|vascular endothelial cadherin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDH5, check out the CDH5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDH5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human VE-Cadherin CDH5 ELISA Kit PicoKine® (EK1317)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human VE-Cadherin CDH5 ELISA Kit PicoKine®
Question
How many reactions can be carried out per unit of EK1317?
Verified customer
Asked: 2022-07-01
Answer
Two replicates of each standard and sample is recommended. One unit of Human VE-Cadherin-5 CDH5 ELISA Kit PicoKine® (EK1317) can be used for testing 40 sample wells in duplicate. 16 wells of standard + 80 wells of samples=96 wells in total
Boster Scientific Support
Answered: 2022-07-01
Question
Q: which procedure should I follow in order to thaw whole blood sample for VE-Cadherin ELISA after freezing?
M. Sunder
Verified customer
Asked: 2020-01-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VE-Cadherin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-29
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement VE-Cadherin using the EK1317 Human VE-Cadherin Picokine® ELISA Kit?
Y. Zhao
Verified customer
Asked: 2018-12-30
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: if the enzyme conjugated VE-Cadherin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VE-Cadherin antigen?
H. Taylor
Verified customer
Asked: 2018-06-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-06-07
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of VE-Cadherin in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VE-Cadherin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-14
Question
Q: What is the optimal O.D. value for VE-Cadherin ELISA kit? I performed your VE-Cadherin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain VE-Cadherin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this VE-Cadherin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of VE-Cadherin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-03-11
Question
I have an unopened package of your VE-Cadherin kit catalog number EK1317. There was some mistake and our shipping team did not mark it as temperature sensitive so it was left at room temperature overnight. Do you think it will still be okay to use this kit after that or will it create serious performance issues? I have moved it to 2-8C. Thank you!
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The performance of the kit will not be affected too much. Please suggest the customer run a pilot test to test the performance. Best Regards,
Boster Scientific Support
Answered: 2017-10-06
Question
Q: is it okay to use citrate plasma as samples in Human VE-Cadherin Picokine® ELISA Kit (Catalog # EK1317)?
Verified Customer
Verified customer
Asked: 2016-11-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of VE-Cadherin causing degradation of its protein structure. VE-Cadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the VE-Cadherin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-11-16
Question
Q: Can VE-Cadherin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-05-27
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-05-27
