Product Info Summary
SKU: | A03222-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Thrombopoietin/Thpo Antibody Picoband®
View all Thrombopoietin/THPO Antibodies
SKU/Catalog Number
A03222-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Thrombopoietin/Thpo Antibody Picoband® catalog # A03222-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombopoietin/Thpo Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03222-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A03222-1 is reactive to Thpo in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
37836 MW
Background of Thrombopoietin/THPO
Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03222-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse liver tissue
IHC: mouse kidney tissue, mouse spleen tissue, rat kidney tissue, mouse liver tissue, rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 4. IHC analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.
Protein Target Info & Infographic
Gene/Protein Information For Thpo (Source: Uniprot.org, NCBI)
Gene Name
Thpo
Full Name
Thrombopoietin
Weight
37836 MW
Superfamily
EPO/TPO family
Alternative Names
Thrombopoietin;C-mpl ligand;ML;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;MGDF;Myeloproliferative leukemia virus oncogene ligand;Thpo; THPO MGDF, MKCSF, ML, MPLLG, THCYT1, TPO thrombopoietin thrombopoietin|MPL ligand|c-mpl ligand|megakaryocyte colony-stimulating factor|megakaryocyte growth and development factor|megakaryocyte stimulating factor|myeloproliferative leukemia virus oncogene ligand|prepro-thrombopoietin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Thpo, check out the Thpo Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Thpo: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thrombopoietin/Thpo Antibody Picoband® (A03222-1)
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