Anti-Thrombopoietin/Thpo Picoband™ Antibody

SKU A03222-1
Size 100μg/vial
Reactivity Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, IHC, WB

Overview

Product Name Anti-Thrombopoietin/Thpo Picoband™ Antibody
SKU/Catalog Number A03222-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.
Cite This Product Anti-Thrombopoietin/Thpo Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03222-1)
Host Rabbit
Contents/Buffer Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Form Lyophilized
Immunogen E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.
Reactivity Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was load

Figure 2. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epi

Figure 3. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, ep

Figure 4. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6

Figure 5. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epit

Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).
Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epit

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P40226
Gene Name Thpo
Protein Name Thrombopoietin
Tissue Specificity Found mainly in the liver, kidney and skeletal muscle.
Alternative Names Thrombopoietin;C-mpl ligand;ML;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;MGDF;Myeloproliferative leukemia virus oncogene ligand;Thpo;
Subcellular Localization Secreted.
Molecular Weight 37836 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Research Areas Mouse, Rat

*You can search these to find other products in these research areas.
Background Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

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In stock
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A03222-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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