Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine®

Cadherin-17 ELISA kit for Mouse

Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CDH17 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1998
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)

Product Name

Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine®

View all Cadherin-17 ELISA kits

SKU/Catalog Number

EK1998

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CDH17 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1998)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: F26-S827

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1998 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1998 is reactive to CDH17 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

EK1998 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cadherin-17

Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. It is mapped to 4; 4 A1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Kit Components

Catalog Number Description Quantity
EK1998-CAP Anti-Mouse CDH17 Pre-coated 96-well strip microplate 1
EK1998-ST Mouse CDH17 Standard 2 vials, 3 ng/tube
EK1998-DA Mouse CDH17 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.993.8187.537575015003000
O.D.0.0580.1510.2130.3350.6250.9491.5072.084

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cadherin-17, Dilution ratio of 1:400, concentration in serum and plasma is around 150 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)903691459913701485
Standard deviation4.4119.5697.755.8221.46117.32
CV (%)4.9%5.3%6.7%6.4%5.8%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cadherin-17 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 190877982844.275.0%
Sample 236938642742340124.536.1%
Sample 31459139913401383139542.663.0%
*number of samples for each test n=16.

Gene/Protein Information For CDH17 (Source: Uniprot.Org, NCBI)

Gene Name

CDH17

Full Name

Cadherin-17

Weight

92.219kDa

Alternative Names

Cadherin-17; BILL-cadherin; Liver-intestine cadherin; LI-cadherin; P130; Cdh17 CDH17 CDH16, HPT-1, HPT1 cadherin 17 cadherin-17|HPT-1 cadherin|LI cadherin|cadherin 17, LI cadherin (liver-intestine)|cadherin-16|human intestinal peptide-associated transporter HPT-1|human peptide transporter 1|intestinal peptide-associated transporter HPT-1|liver-intestine cadherin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDH17, check out the CDH17 Infographic

CDH17 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDH17: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-18

Question

Q: how do I analyze ELISA data? I have performed Cadherin-17 level in plasma.

N. Jha

Verified customer

Asked: 2020-01-23

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-01-23

Question

Q: is it okay to use citrate plasma as samples in Mouse Cadherin-17 Picokine® ELISA Kit (Catalog # EK1998)?

Verified Customer

Verified customer

Asked: 2019-08-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Cadherin-17 causing degradation of its protein structure. Cadherin-17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cadherin-17 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-08-24

Question

Q: if the enzyme conjugated Cadherin-17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cadherin-17 antigen?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-08-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-03-31

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-31

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Cadherin-17 using the EK1998 Mouse Cadherin-17 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-06-19

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2017-06-19

Question

Q: can you suggest the dilution ratio of serum samples for detection of Cadherin-17 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

N. Mitchell

Verified customer

Asked: 2017-02-15

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cadherin-17 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-02-15

Question

Q: Can Cadherin-17 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

J. King

Verified customer

Asked: 2014-08-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-08-23

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