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Product Info Summary
| SKU: | PB10102 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Thrombopoietin/THPO Antibody Picoband®
View all Thrombopoietin/THPO Antibodies
SKU/Catalog Number
PB10102
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # PB10102. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombopoietin/THPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10102)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Thrombopoietin/TPO recombinant protein (Position: S22-R258). Human Thrombopoietin/TPO shares 79.3% and 80.6% amino acid (aa) sequence identity with mouse and rat Thrombopoietin/TPO, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10102 is reactive to THPO in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
37823 MW
Background of Thrombopoietin/THPO
Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10102 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Thrombopoietin/TPO using anti-Thrombopoietin/TPO antibody (PB10102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin/TPO antigen affinity purified polyclonal antibody (Catalog # PB10102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin/TPO at approximately 45 kDa. The expected band size for Thrombopoietin/TPO is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For THPO (Source: Uniprot.org, NCBI)
Gene Name
THPO
Full Name
Thrombopoietin
Weight
37823 MW
Superfamily
EPO/TPO family
Alternative Names
Thrombopoietin;C-mpl ligand;ML;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;MGDF;Myeloproliferative leukemia virus oncogene ligand;THPO;MGDF; THPO MGDF, MKCSF, ML, MPLLG, THCYT1, TPO thrombopoietin thrombopoietin|MPL ligand|c-mpl ligand|megakaryocyte colony-stimulating factor|megakaryocyte growth and development factor|megakaryocyte stimulating factor|myeloproliferative leukemia virus oncogene ligand|prepro-thrombopoietin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THPO, check out the THPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thrombopoietin/THPO Antibody Picoband® (PB10102)
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Customer Q&As
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4 Customer Q&As for Anti-Thrombopoietin/THPO Antibody Picoband®
Question
We are currently using anti-Thrombopoietin/THPO antibody PB10102 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The anti-Thrombopoietin/THPO antibody (PB10102) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-27
Question
I see that the anti-Thrombopoietin/THPO antibody PB10102 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-12
Question
Does anti-Thrombopoietin/THPO antibody PB10102 work on primate ELISA with fetal liver?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not validated anti-Thrombopoietin/THPO antibody PB10102 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Thrombopoietin/THPO antibody PB10102 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate fetal liver in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Is this PB10102 anti-Thrombopoietin/THPO antibody reactive to the isotypes of THPO?
E. Carter
Verified customer
Asked: 2019-06-17
Answer
The immunogen of PB10102 anti-Thrombopoietin/THPO antibody is E. coli-derived human Thrombopoietin/TPO recombinant protein (Position: S22-R258). Human Thrombopoietin/TPO shares 79.3% and 80.6% amino acid (aa) sequence identity with mouse and rat Thrombopoietin/TPO, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-17
