Product Info Summary
SKU: | PA2200 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-LI-Cadherin-17 CDH17-Antibody Picoband®
View all Cadherin-17 Antibodies
SKU/Catalog Number
PA2200
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LI-Cadherin-17 CDH17-Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LI-Cadherin-17 CDH17-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2200)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LI Cadherin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2200 is reactive to CDH17 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
92219 MW
Background of Cadherin-17
Cadherin-17, also known as HPT1 or CDH16 is a protein that in humans is encoded by the CDH17 gene. By somatic cell hybrid analysis and fluorescence in situ hybridization, CDH17 gene is mapped to 8q22.1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2200 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, SW620 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-CDH17 antibody, PA2200, Western blotting
All lanes: Anti CDH17(PA2200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
Protein Target Info & Infographic
Gene/Protein Information For CDH17 (Source: Uniprot.org, NCBI)
Gene Name
CDH17
Full Name
Cadherin-17
Weight
92219 MW
Alternative Names
Cadherin-17;Intestinal peptide-associated transporter HPT-1;Liver-intestine cadherin;LI-cadherin;CDH17; CDH17 CDH16, HPT-1, HPT1 cadherin 17 cadherin-17|HPT-1 cadherin|LI cadherin|cadherin 17, LI cadherin (liver-intestine)|cadherin-16|human intestinal peptide-associated transporter HPT-1|human peptide transporter 1|intestinal peptide-associated transporter HPT-1|liver-intestine cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH17, check out the CDH17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LI-Cadherin-17 CDH17-Antibody Picoband® (PA2200)
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No publications found for PA2200
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-LI-Cadherin-17 CDH17-Antibody Picoband®
Question
I was wanting to use your anti-LI Cadherin/CDH17 antibody for WB for human colonic mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colonic mucosa identification?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It shows on the product datasheet, PA2200 anti-LI Cadherin/CDH17 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colonic mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
Is a blocking peptide available for product anti-LI Cadherin/CDH17 antibody (PA2200)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do provide the blocking peptide for product anti-LI Cadherin/CDH17 antibody (PA2200). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-12
Question
Will PA2200 anti-LI Cadherin/CDH17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Johnson
Verified customer
Asked: 2019-06-06
Answer
You can see on the product datasheet, PA2200 anti-LI Cadherin/CDH17 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-LI Cadherin/CDH17 antibody PA2200 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
B. Thomas
Verified customer
Asked: 2014-05-12
Answer
The anti-LI Cadherin/CDH17 antibody (PA2200) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-12