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Key=updated_at, value=2024-10-19 05:03:20
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Key=name, value=Anti-LI-Cadherin-17 CDH17-Antibody Picoband®
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Key=meta_description, value=Boster Bio Anti-LI-Cadherin-17 CDH17 Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Anti- CDH17 antibody, PA2200, Western blotting
All lanes: Anti CDH17(PA2200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
Key=small_image_label, value=Anti- CDH17 antibody, PA2200, Western blotting
All lanes: Anti CDH17(PA2200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
Key=thumbnail_label, value=Anti- CDH17 antibody, PA2200, Western blotting
All lanes: Anti CDH17(PA2200) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
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Key=size, value=100 μg/vial
Key=protein_name, value=Cadherin-17
Key=gene_full_name, value=Cadherin-17
Key=uniprot_id, value=Q12864
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=applications, value=WB
Key=reactivity, value=Human
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=source_company, value=Boster PA2200
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=92219 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Blood Circulation:A05505^Peptide Transport:A05292-1|PB9823|PB9824|A01496
Key=description, value=Boster Bio Anti-LI-Cadherin-17 CDH17-Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-LI-Cadherin-17 CDH17-Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-LI Cadherin/CDH17 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Cadherin-17, also known as HPT1 or CDH16 is a protein that in humans is encoded by the CDH17 gene. By somatic cell hybrid analysis and fluorescence in situ hybridization, CDH17 gene is mapped to 8q22.1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
Key=gene_name, value=CDH17
Key=synonyms, value=Cadherin-17;Intestinal peptide-associated transporter HPT-1;Liver-intestine cadherin;LI-cadherin;CDH17;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human

Key=subcellular_localization, value= Cell membrane ; Single-pass type I membrane protein .
Key=sequence_similarities, value=Contains 7 cadherin domains.
Key=tissue_specificity, value= Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin. .
Key=protein_function, value= Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport. .
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 25628|sc 393045|sc 393533|sc 59876|sc 6978|sc 74209
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human LI Cadherin.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=92 kDa
Key=custom_attribute_2, value=WB: HELA Whole Cell, SW620 Whole Cell
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Anti-LI-Cadherin-17 CDH17-Antibody Picoband®

Cadherin-17 antibody

Boster Bio Anti-LI-Cadherin-17 CDH17-Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2200
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-LI-Cadherin-17 CDH17-Antibody Picoband®

View all Cadherin-17 Antibodies

SKU/Catalog Number

PA2200

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LI-Cadherin-17 CDH17-Antibody catalog # PA2200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LI-Cadherin-17 CDH17-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2200)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LI Cadherin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2200 is reactive to CDH17 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

92 kDa

Calculated molecular weight

92219 MW

Background of Cadherin-17

Cadherin-17, also known as HPT1 or CDH16 is a protein that in humans is encoded by the CDH17 gene. By somatic cell hybrid analysis and fluorescence in situ hybridization, CDH17 gene is mapped to 8q22.1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2200 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Whole Cell, SW620 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For CDH17 (Source: Uniprot.org, NCBI)

Gene Name

CDH17

Full Name

Cadherin-17

Weight

92219 MW

Alternative Names

Cadherin-17;Intestinal peptide-associated transporter HPT-1;Liver-intestine cadherin;LI-cadherin;CDH17; CDH17 CDH16, HPT-1, HPT1 cadherin 17 cadherin-17|HPT-1 cadherin|LI cadherin|cadherin 17, LI cadherin (liver-intestine)|cadherin-16|human intestinal peptide-associated transporter HPT-1|human peptide transporter 1|intestinal peptide-associated transporter HPT-1|liver-intestine cadherin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDH17, check out the CDH17 Infographic

CDH17 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDH17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-LI-Cadherin-17 CDH17-Antibody Picoband®

Question

I was wanting to use your anti-LI Cadherin/CDH17 antibody for WB for human colonic mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colonic mucosa identification?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

It shows on the product datasheet, PA2200 anti-LI Cadherin/CDH17 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colonic mucosa in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-29

Question

Is a blocking peptide available for product anti-LI Cadherin/CDH17 antibody (PA2200)?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

We do provide the blocking peptide for product anti-LI Cadherin/CDH17 antibody (PA2200). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-12

Question

Will PA2200 anti-LI Cadherin/CDH17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Johnson

Verified customer

Asked: 2019-06-06

Answer

You can see on the product datasheet, PA2200 anti-LI Cadherin/CDH17 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-06

Question

We are currently using anti-LI Cadherin/CDH17 antibody PA2200 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

B. Thomas

Verified customer

Asked: 2014-05-12

Answer

The anti-LI Cadherin/CDH17 antibody (PA2200) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-05-12

Order DetailsPrice
PA2200

100μg

$370
PA2200-10ug

10μg sample (liquid)

$99
PA2200-Biotin

100 μg Biotin conjugated

$570
PA2200-Cy3

100 μg Cy3 conjugated

$570
PA2200-Dylight488

100 μg Dylight488 conjugated

$570
PA2200-Dylight550

100 μg Dylight550 conjugated

$570
PA2200-Dylight594

100 μg Dylight594 conjugated

$570
PA2200-FITC

100 μg FITC conjugated

$570
PA2200-HRP

100 μg HRP conjugated

$570
PA2200-APC

100 μg APC conjugated

$670
PA2200-PE

100 μg PE conjugated

$670
PA2200-iFluor647

100 μg iFluor647 conjugated

$670
PA2200-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2200
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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