Key=store_id
Key=entity_id, value=157
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Key=type_id, value=simple
Key=sku, value=EK0517
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Key=created_at, value=2014-05-19 10:17:44
Key=updated_at, value=2024-09-26 00:43:57
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Mouse Thrombopoietin/TPO ELISA Kit PicoKine®
Key=cj_search_index
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Key=is_recurring, value=No
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Key=template, value=elisa-kits
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Key=yoast_content_score, value=60
Key=price, value=499.0000
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Key=meta_title, value=Mouse Thrombopoietin/TPO ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Mouse Thrombopoietin/TPO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Thpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 5pg/ml.
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Key=url_key, value=mouse-thrombopoietin-tpo-picokine-trade-elisa-kit-ek0517-boster
Key=url_path, value=mouse-thrombopoietin-tpo-picokine-trade-elisa-kit-ek0517-boster
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Key=image_label, value=Mouse TPO PicoKine ELISA Kit standard curve
Key=small_image_label, value=Mouse TPO PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Mouse TPO PicoKine ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Thrombopoietin
Key=gene_full_name, value=Thrombopoietin
Key=uniprot_id, value=P40226
Key=assay_range, value=62.5 pg/ml - 4,000 pg/ml
Key=sensitivity, value=<30 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Mouse
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=monoclonal antibody from rat|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=normal_range_citations, value=12578637
Key=predicted_reactivity
Key=source_company, value=Boster EK0517
Key=molecular_weight, value=37836 MW
Key=related_product_skus, value=PROTP07202
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Zinc Finger
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Key=rating_value, value=100
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Key=cj_related_products, value=Amino Acid Transport:PB9959|A02133^Chloride Transport:A04360-2^Myoclonus:A10622-2^Thrombocytosis:M00176-5^Transepithelial Transport:EK1998
Key=description, value=Mouse Thrombopoietin/TPO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Thpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse Thrombopoietin/TPO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Thpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Mouse TPO ELISA Kit, Thrombopoietin ELISA Kit, Bosterbio High Sensitivity
Key=background, value=Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
Key=gene_name, value=Thpo
Key=synonyms, value=Thrombopoietin;C-mpl ligand;ML;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;MGDF;Myeloproliferative leukemia virus oncogene ligand;Thpo;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	92	307	851	85	330	918
Standard deviation	6.53	20.87	47.65	6.8	27.39	55.08
CV (%)	7.1%	6.8%	5.6%	8%	8.3%	6%

Key=application_details, value=Dilution ratio of 1:10, concentration in serum and plasma is around 6 ng/ml.
Key=subcellular_localization, value= Secreted.
Key=tissue_specificity, value= Found mainly in the liver, kidney and skeletal muscle.
Key=protein_function, value= Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Key=sample_data, value=

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.055	0.098	0.124	0.215	0.399	0.768	1.468	2.036

Key=competitor_equivalent_skus, value=sc 1299|sc 374045|sc 1300|sc 1298|sc 366264|sc 366265|sc 398533|sc 398525|sc 514326
Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: S22-T356
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	92	87	100	93	93	4.63	4.9%
Sample 2	307	267	309	301	296	17	5.7%
Sample 3	851	915	849	807	855	38.58	4.5%

*number of samples for each test n=16.
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