Key=store_id
Key=entity_id, value=54635
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EKC2199
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2019-02-12 00:21:00
Key=updated_at, value=2024-09-26 01:01:51
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit
Key=cj_search_index
Key=publication_count, value=0
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=cell-based-phospho-elisa-kits
Key=price, value=540.0000
Key=weight, value=1.0000
Key=meta_title, value=p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit Price
Key=meta_description, value=p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit EKC2199
Key=image, value=/b/o/boster-box.png
Key=small_image, value=/b/o/boster-box.png
Key=thumbnail, value=/b/o/boster-box.png
Key=url_key, value=p38-mapk-phospho-thr179-tyr181-colorimetric-cell-based-elisa-kit-ekc2199-boster
Key=url_path, value=p38-mapk-phospho-thr179-tyr181-colorimetric-cell-based-elisa-kit-ekc2199-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Boster Kit Box
Key=small_image_label, value=Boster Kit Box
Key=thumbnail_label, value=Boster Kit Box
Key=msrp_display_actual_price_type, value=
Key=size, value=1 kit, containing two 96-well plates and all necessary reagents
Key=gene_full_name, value=Mitogen-activated protein kinase 14
Key=uniprot_id, value=Q16539
Key=assay_range, value=> 5000 cells/well
Key=sensitivity
Key=applications, value=ELISA
Key=reactivity, value=Human, Mouse, Rat
Key=source_company, value=AssayBiotech CBP1304; Cost: 300
Key=molecular_weight, value=41293 MW
Key=product_category, value=Cell-Based ELISA Kits
Key=rating_value, value=100
Key=review_count, value=13
Key=cj_related_products, value=Myeloproliferative Disease:PB10102^Oocyte Maturation:A16237^Thrombocytosis:P00176-1|A11022-1|EK0517|PB10102
Key=special_from_date
Key=description, value=The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).
Key=short_description, value=The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).
Key=gene_name, value=MAPK14
Key=synonyms, value=protein;kinase p38-alpha;MAP kinase MXI2;MAP kinase p38;MAPK14;MAX-interacting protein 2;Mitogen-activated protein kinase 14;Mitogen-activated protein kinase p38;MK14;MXI2
Key=sample_type, value=Cell lines
Key=kit_components, value=p38 MAPK (Phospho-Thr179+Tyr181)
Key=subcellular_localization, value=Cytoplasm. Nucleus.
Key=tissue_specificity, value=Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Key=protein_function, value= Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF- induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14- mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF- kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.
Key=storage, value=Store at 4°C for up to 6 months.
Key=custom_attribute_1, value=p38 MAPK
Key=custom_attribute_2, value=Phospho-Thr179+Tyr181
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=quantity_and_stock_status
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images

p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

p38 alpha ELISA kit, phospho-specific, cell-based

The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).

Product Info Summary

SKU: EKC2199
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

See all p38 alpha products

SKU/Catalog Number

EKC2199

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2199)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2199 is reactive to MAPK14 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2199 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-p38 MAPK Phospho-Thr179+Tyr181 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-p38 MAPK Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For MAPK14 (Source: Uniprot.Org, NCBI)

Gene Name

MAPK14

Full Name

Mitogen-activated protein kinase 14

Weight

41293 MW

Superfamily

protein kinase superfamily

Alternative Names

protein;kinase p38-alpha;MAP kinase MXI2;MAP kinase p38;MAPK14;MAX-interacting protein 2;Mitogen-activated protein kinase 14;Mitogen-activated protein kinase p38;MK14;MXI2 MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAPK14, check out the MAPK14 Infographic

MAPK14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAPK14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC2199

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
In stock
Order Product
EKC2199
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.