p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

p38 alpha ELISA kit, phospho-specific, cell-based

The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).

Product Info Summary

SKU: EKC2199
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit

See all p38 alpha products

SKU/Catalog Number

EKC2199

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The p38 MAPK (Phospho-Thr179+Tyr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Thr179+Tyr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of p38 MAPK (Phospho-Thr179+Tyr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p38 MAPK (Phospho-Thr179+Tyr181).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

p38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2199)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2199 is reactive to MAPK14 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2199 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-p38 MAPK Phospho-Thr179+Tyr181 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-p38 MAPK Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For MAPK14 (Source: Uniprot.Org, NCBI)

Gene Name

MAPK14

Full Name

Mitogen-activated protein kinase 14

Weight

41293 MW

Superfamily

protein kinase superfamily

Alternative Names

protein;kinase p38-alpha;MAP kinase MXI2;MAP kinase p38;MAPK14;MAX-interacting protein 2;Mitogen-activated protein kinase 14;Mitogen-activated protein kinase p38;MK14;MXI2 MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAPK14, check out the MAPK14 Infographic

MAPK14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAPK14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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