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Product Info Summary
| SKU: | P02109 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-Shb (Tyr246) Antibody
SKU/Catalog Number
P02109
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Shb (Tyr246) Antibody (Catalog # P02109). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Shb (Tyr246) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02109)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human Shb around the phosphorylation site of Y246.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02109 is reactive to SHB in Human, Mouse
Reconstitution
Calculated molecular weight
55042 MW
Background of SHB
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02109 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
ELISA: 1/10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of 293 cells using Phospho-Shb (Y246) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For SHB (Source: Uniprot.org, NCBI)
Gene Name
SHB
Full Name
SH2 domain-containing adapter protein B
Weight
55042 MW
Alternative Names
SH2 domain-containing adapter protein B;SHB; SHB bA3J10.2 SH2 domain containing adaptor protein B SH2 domain-containing adapter protein B|SHB (Src homology 2 domain containing) adaptor protein B|SHB adaptor protein (a Src homology 2 protein)|Src homology 2 domain containing adaptor protein B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHB, check out the SHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Shb (Tyr246) Antibody (P02109)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-Shb (Tyr246) Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
Does anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of liver, SHB is highly expressed in liver. So, it is likely that anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 will work for WB with liver.
Boster Scientific Support
Answered: 2020-02-11
Question
I see that the anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-11
Question
Will anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 work on feline WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not validated anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
Is this P02109 anti-Phospho-Shb (Tyr246) Polyclonal antibody reactive to the isotypes of SHB?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The immunogen of P02109 anti-Phospho-Shb (Tyr246) Polyclonal antibody is Synthesized peptide derived from human Shb around the phosphorylation site of Y246. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-Phospho-Shb (Tyr246) Polyclonal antibody P02109 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-Phospho-Shb (Tyr246) Polyclonal antibody (P02109) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
Question
I have a question about product P02109, anti-Phospho-Shb (Tyr246) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Lewis
Verified customer
Asked: 2015-10-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02109 anti-Phospho-Shb (Tyr246) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-10-13
