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Product Info Summary
| SKU: | A02109-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HUMAN-SHB(Y268) Antibody
SKU/Catalog Number
A02109-1
Size
80 µl
Description
Boster Bio Anti-HUMAN-SHB(Y268) Antibody (Catalog # A02109-1). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HUMAN-SHB(Y268) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02109-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 250-290 amino acids from human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02109-1 is reactive to SHB in Human
Reconstitution
Calculated molecular weight
55042 Da
Background of SHB
Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic- FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02109-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from HepG2, Jurkat, K562, HUVEC cell line (from left to right), using SHB-Y268 Antibody. A02109-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Immunohistochemical analysis of paraffin-embedded H. small intestine section using Phospho-HUMAN-SHB(Y268). ctrl. A02109-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of HepG2 cells using Phospho-HUMAN-SHB (Y268). ctrl(green, Cat#A02109-1) compared to an isotype control of rabbit IgG(blue). A02109-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For SHB (Source: Uniprot.org, NCBI)
Gene Name
SHB
Full Name
SH2 domain-containing adapter protein B
Weight
55042 Da
Alternative Names
SH2 domain-containing adapter protein B, SHB SHB bA3J10.2 SH2 domain containing adaptor protein B SH2 domain-containing adapter protein B|SHB (Src homology 2 domain containing) adaptor protein B|SHB adaptor protein (a Src homology 2 protein)|Src homology 2 domain containing adaptor protein B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHB, check out the SHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HUMAN-SHB(Y268) Antibody (A02109-1)
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