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Product Info Summary
| SKU: | EK0975 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®
View all Chitinase 3-like 1 ELISA kits
SKU/Catalog Number
EK0975
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Chi3l1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0975)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y22-A381
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0975 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0975 is reactive to Chi3l1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0975 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chitinase 3-like 1
Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0975-CAP | Anti-Mouse Chi3l1 Pre-coated 96-well strip microplate | 1 |
| EK0975-ST | Mouse Chi3l1 Standard | 2 vials, 10 ng/tube |
| EK0975-DA | Mouse Chi3l1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.019 | 0.078 | 0.129 | 0.249 | 0.421 | 1.030 | 1.198 | 1.802 |
Data Example Images
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Mouse Chitinase 3-like 1/YKL-40 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Chitinase 3-like 1, Dilution ratio of 1:100, concentration in serum and plasma is around 100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 176 | 647 | 1183 | 170 | 667 | 1185 |
| Standard deviation | 9.68 | 32.99 | 81.62 | 12.58 | 43.35 | 103.09 |
| CV (%) | 5.5% | 8.7% | 6.9% | 7.4% | 6.5% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chitinase 3-like 1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 176 | 161 | 162 | 178 | 169 | 7.79 | 4.6% |
| Sample 2 | 647 | 638 | 670 | 714 | 667 | 29.4 | 4.4% |
| Sample 3 | 1183 | 1204 | 1258 | 1324 | 1242 | 54.55 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For Chi3l1 (Source: Uniprot.Org, NCBI)
Gene Name
Chi3l1
Full Name
Chitinase-3-like protein 1
Weight
43893 MW
Superfamily
glycosyl hydrolase 18 family
Alternative Names
Chitinase-3-like protein 1;BRP39 protein;Breast regression protein 39;Cartilage glycoprotein 39;CGP-39;GP-39;Chi3l1;Brp39, Chil1; CHI3L1 ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39 chitinase 3 like 1 chitinase-3-like protein 1|39 kDa synovial protein|cartilage glycoprotein 39|chitinase 3-like 1 (cartilage glycoprotein-39)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Chi3l1, check out the Chi3l1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Chi3l1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine® (EK0975)
Hello CJ!
EK0975 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
YKL-40 in the brain and cerebrospinal fluid of neurodegenerative dementias
Adenovirus vector-mediated YKL-40 shRNA attenuates eosinophil airway inflammation in a murine asthmatic model
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for Chitinase 3-like 1 ELISA kit? I performed your Chitinase 3-like 1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Chitinase 3-like 1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-06-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Chitinase 3-like 1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Chitinase 3-like 1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-06-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-28
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-28
Question
Would EK1172, EK0385 and EK0975 work for mouse tissue homogenates?
Verified customer
Asked: 2019-10-14
Answer
Although the Mouse FGF1/Fgf Acidic ELISA Kit PicoKine EK1172, Mouse IGFBP2 ELISA Kit PicoKine® (EK0385) and Mouse Chitinase 3-Like 1/YKL-40 ELISA Kit PicoKine® (EK0975) has not been validated for mouse tissue homogenates samples, the ELISA kit will work for all sample types as long as target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot tests to decide the optimal sample dilution. For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit: • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-10-15
Question
Would EK0975 work for mouse tissue homogenates?
Verified customer
Asked: 2019-10-14
Answer
Although the Mouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine EK0975 has not been validated for mouse tissue homogenates samples, the ELISA kit will work for all sample types as long as target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot tests to decide the optimal sample dilution. For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit: • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-10-15
Question
What is the exact sensitivity of EK0975?
Verified customer
Asked: 2019-09-18
Answer
The sensitivity of the Mouse Chitinase 3-Like 1/YKL-40 ELISA Kit PicoKine™ (EK0975) is <10pg/ml.
Boster Scientific Support
Answered: 2019-09-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-04-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-21
Question
Q: can you recommend the dilution ratio of serum samples for detection of Chitinase 3-like 1 in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Chitinase 3-like 1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-24
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
P. Sunder
Verified customer
Asked: 2018-04-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-26
