Anti-ME3 Antibody

Product Name

Anti-ME3 Antibody

View all ME3 Antibodies

SKU/Catalog Number

A05164

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ME3 Antibody (Catalog # A05164). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ME3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05164)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ME3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05164 is reactive to ME3 in Human, Mouse

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

67068 MW

Background of ME3

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Loeber G., Biochem. J. 304:687-692(1994).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A05164 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For ME3 (Source: Uniprot.org, NCBI)

Alternative Names

NADP-dependent malic enzyme, mitochondrial;NADP-ME;1.1.1.40;Malic enzyme 3;ME3; ME3 NADP-ME malic enzyme 3 NADP-dependent malic enzyme, mitochondrial|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))|malic enzyme 3, NADP(+)-dependent, mitochondrial|malic enzyme, NADP+-dependent, mitochondrial|mitochondrial NADP(+)-dependent malic enzyme 3|pyruvic-malic carboxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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