Anti-PD1 PDCD1 Monoclonal Antibody [4D6]

Product Name

Anti-PD1 PDCD1 Monoclonal Antibody [4D6]

View all PD-1 Antibodies

SKU/Catalog Number

M00178-4

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PD1 PDCD1 Monoclonal Antibody [4D6] (Catalog # M00178-4). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PD1 PDCD1 Monoclonal Antibody [4D6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00178-4)

Host

Mouse

Contents

PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 4D6

Isotype

IgG1

Immunogen

PD-1 antibody was raised against the extracellular domain of human PD-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PD-L1 Antibody has no cross-reactivity to PD-L2.

Reactive Species

M00178-4 is reactive to PDCD1 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

31647 MW

Background of PD-1

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00178-4 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PD-1 antibody can be used for detection of PD-1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PDCD1 (Source: Uniprot.org, NCBI)

Alternative Names

PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1 PDCD1 CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 programmed cell death 1 programmed cell death protein 1|programmed cell death 1 protein|protein PD-1|systemic lupus erythematosus susceptibility 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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